BRENDA - Enzyme Database
show all sequences of 1.5.1.B5

Ornithine cyclodeaminase/mu-crystallin homolog from the hyperthermophilic archaeon Thermococcus litoralis functions as a novel DELTA(1)-pyrroline-2-carboxylate reductase involved in putative trans-3-hydroxy-L-proline metabolism

Watanabe, S.; Tozawa, Y.; Watanabe, Y.; FEBS Open Bio 4, 617-626 (2014)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
His-tagged protein is expressed in Escherichia coli BL21. TlLhpI is a fusion of OCC_00362 and OCC_00367 genes by PCR. The gene from Thermococcus litoralis DSM 5473 consists of two separate open-reading frames (ORFs): OCC_00362 and OCC_00367. When a single A is inserted between A227646 and T227647 in the original genome sequence of T. litoralis DSM 5473, the separate ORFs fuse into a single ORF, and the putative amino acid sequence of is homologous to that of the Thermococcus sibiricus TSIB_0634 gene. Although there is a possibility that this OCD-like protein from Thermococcus litoralis DSM 5473 forms a heterooligomeric structure, the TlLhpI gene is synthesized as a single ORF by sequential steps of PCR using sense and antisense primers and genome DNA of Thermococcus litoralis DSM 5473 as a template
Thermococcus litoralis
Engineering
Protein Variants
Commentary
Organism
L52E/V224D/A228K
DELTA1-pyrroline-2-carboxylate reductase activity is the same as in wild-type enzyme, no alanine dehydrogenase activity
Thermococcus litoralis
L52R/V224D/A228K
DELTA1-pyrroline-2-carboxylate reductase activity is the same as in wild-type enzyme, no alanine dehydrogenase activity
Thermococcus litoralis
additional information
TlLhpI is a fusion of OCC_00362 and OCC_00367 genes by PCR. The gene from Thermococcus litoralis DSM 5473 consistsof two separate open-reading frames (ORFs): OCC_00362 and OCC_00367. When a single A is inserted between A227646 and T227647 in the original genome sequence of T. litoralis DSM 5473, the separate ORFs fuse into a single ORF, and the putative amino acid sequence of is homologous to that of the Thermococcus sibiricus TSIB_0634 gene. Although there is a possibility that this OCD-like protein from Thermococcus litoralis DSM 5473 forms a heterooligomeric structure, the TlLhpI gene is synthesized as a single ORF by sequential steps of PCR using sense and antisense primers and genome DNA of Thermococcus litoralis DSM 5473 as a template
Thermococcus litoralis
V224D/A228K
12fold enhancement of DELTA1-pyrroline-2-carboxylate reductase activity, 6.7fold enhancement for the oxidization activity for L-proline, no alanine dehydrogenase activity
Thermococcus litoralis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.444
-
L-proline
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis
0.608
-
L-pipecolate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
0.944
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
1.12
-
L-proline
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
2.77
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Thermococcus litoralis
72000
-
gel filtration
Thermococcus litoralis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
DELTA1-pyrroline-2-carboxylate + NADH + H+
Thermococcus litoralis
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
L-proline + NAD+
-
-
?
DELTA1-pyrroline-2-carboxylate + NADH + H+
Thermococcus litoralis DSM 5473
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
L-proline + NAD+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Thermococcus litoralis
H3ZMH3 and H3ZMH4
TlLhpI is a fusion of OCC_00362 and OCC_00367 genes by PCR. The gene from Thermococcus litoralis DSM 5473 consists of two separate open-reading frames (ORFs): OCC_00362 and OCC_00367. When a single A is inserted between A227646 and T227647 in the original genome sequence of T. litoralis DSM 5473, the separate ORFs fuse into a single ORF, and the putative amino acid sequence of is homologous to that of the Thermococcus sibiricus TSIB_0634 gene. Although there is a possibility that this OCD-like protein from Thermococcus litoralis DSM 5473 forms a heterooligomeric structure, the TlLhpI gene is synthesized as a single ORF by sequential steps of PCR using sense and antisense primers and genome DNA of Thermococcus litoralis DSM 5473 as a template
-
Thermococcus litoralis DSM 5473
H3ZMH3 and H3ZMH4
TlLhpI is a fusion of OCC_00362 and OCC_00367 genes by PCR. The gene from Thermococcus litoralis DSM 5473 consists of two separate open-reading frames (ORFs): OCC_00362 and OCC_00367. When a single A is inserted between A227646 and T227647 in the original genome sequence of T. litoralis DSM 5473, the separate ORFs fuse into a single ORF, and the putative amino acid sequence of is homologous to that of the Thermococcus sibiricus TSIB_0634 gene. Although there is a possibility that this OCD-like protein from Thermococcus litoralis DSM 5473 forms a heterooligomeric structure, the TlLhpI gene is synthesized as a single ORF by sequential steps of PCR using sense and antisense primers and genome DNA of Thermococcus litoralis DSM 5473 as a template
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Thermococcus litoralis
Reaction
Reaction
Commentary
Organism
Reaction ID
L-pipecolate + NAD+ = DELTA1-piperideine-2-carboxylate + NADH + H+
(1)
Thermococcus litoralis
L-proline + NAD+ = DELTA1-pyrroline-2-carboxylate + NADH + H+
(2)
Thermococcus litoralis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
cis-4-hydroxy-L-proline + NAD+
activity is 85% compared to the activity with L-proline
729750
Thermococcus litoralis
?
-
-
-
?
cis-4-hydroxy-L-proline + NAD+
activity is 85% compared to the activity with L-proline
729750
Thermococcus litoralis DSM 5473
?
-
-
-
?
DELTA1-pyrroline-2-carboxylate + NADH + H+
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
729750
Thermococcus litoralis
L-proline + NAD+
-
-
-
?
DELTA1-pyrroline-2-carboxylate + NADH + H+
preference of the reaction equilibrium in the direction toward NADH-dependent reduction
729750
Thermococcus litoralis
L-proline + NAD+
-
-
-
r
DELTA1-pyrroline-2-carboxylate + NADH + H+
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
729750
Thermococcus litoralis DSM 5473
L-proline + NAD+
-
-
-
?
DELTA1-pyrroline-2-carboxylate + NADH + H+
preference of the reaction equilibrium in the direction toward NADH-dependent reduction
729750
Thermococcus litoralis DSM 5473
L-proline + NAD+
-
-
-
r
L-pipecolate + NAD+
activity is 49% compared to the activity with L-proline
729750
Thermococcus litoralis
DELTA1-piperideine-2-carboxylate + NADH + H+
-
-
-
?
L-pipecolate + NAD+
activity is 49% compared to the activity with L-proline
729750
Thermococcus litoralis DSM 5473
DELTA1-piperideine-2-carboxylate + NADH + H+
-
-
-
?
L-proline + NAD+
preference of the reaction equilibrium in the direction toward NADH-dependent reduction
729750
Thermococcus litoralis
DELTA1-pyrroline-2-carboxylate + NADH + H+
-
-
-
r
additional information
the enzyme also shows alanine dehydrogenase activity (EC 1.4.1.1) and N-methyl-L-alanine dehydrogenase activity (EC 1.4.1.17)
729750
Thermococcus litoralis
?
-
-
-
-
additional information
the enzyme also shows alanine dehydrogenase activity (EC 1.4.1.1) and N-methyl-L-alanine dehydrogenase activity (EC 1.4.1.17)
729750
Thermococcus litoralis DSM 5473
?
-
-
-
-
trans-4-hydroxy-L-proline + NAD+
activity is 70% compared to the activity with L-proline
729750
Thermococcus litoralis
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE
Thermococcus litoralis
Synonyms
Synonyms
Commentary
Organism
DELTA1-pyrroline-2-carboxylate reductase
misleading
Thermococcus litoralis
Pyr2C reductase
-
Thermococcus litoralis
TlLhpI
-
Thermococcus litoralis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
assay at
Thermococcus litoralis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.2
-
L-pipecolate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
0.23
-
L-proline
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
0.61
-
L-proline
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis
16.6
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
558
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4
6
substrates: DELTA1-pyrroline-2-carboxylate + NADH + H+
Thermococcus litoralis
11
-
substrates: L-proline + NAD+
Thermococcus litoralis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
11
pH 5.0: about 40% of maximal activity, pH 11.0: about 80% of maximal activity
Thermococcus litoralis
10
12
pH 10.0: about 75% of maximal activity, pH 12.0: about 85% of maximal activity
Thermococcus litoralis
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
the kcat/Km value for proline in the presence of NADP+ is about 50fold lower than that in the presence of NAD+
Thermococcus litoralis
NADH
no activity with NADPH
Thermococcus litoralis
Cloned(Commentary) (protein specific)
Commentary
Organism
His-tagged protein is expressed in Escherichia coli BL21. TlLhpI is a fusion of OCC_00362 and OCC_00367 genes by PCR. The gene from Thermococcus litoralis DSM 5473 consists of two separate open-reading frames (ORFs): OCC_00362 and OCC_00367. When a single A is inserted between A227646 and T227647 in the original genome sequence of T. litoralis DSM 5473, the separate ORFs fuse into a single ORF, and the putative amino acid sequence of is homologous to that of the Thermococcus sibiricus TSIB_0634 gene. Although there is a possibility that this OCD-like protein from Thermococcus litoralis DSM 5473 forms a heterooligomeric structure, the TlLhpI gene is synthesized as a single ORF by sequential steps of PCR using sense and antisense primers and genome DNA of Thermococcus litoralis DSM 5473 as a template
Thermococcus litoralis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
the kcat/Km value for proline in the presence of NADP+ is about 50fold lower than that in the presence of NAD+
Thermococcus litoralis
NADH
no activity with NADPH
Thermococcus litoralis
Engineering (protein specific)
Protein Variants
Commentary
Organism
L52E/V224D/A228K
DELTA1-pyrroline-2-carboxylate reductase activity is the same as in wild-type enzyme, no alanine dehydrogenase activity
Thermococcus litoralis
L52R/V224D/A228K
DELTA1-pyrroline-2-carboxylate reductase activity is the same as in wild-type enzyme, no alanine dehydrogenase activity
Thermococcus litoralis
additional information
TlLhpI is a fusion of OCC_00362 and OCC_00367 genes by PCR. The gene from Thermococcus litoralis DSM 5473 consistsof two separate open-reading frames (ORFs): OCC_00362 and OCC_00367. When a single A is inserted between A227646 and T227647 in the original genome sequence of T. litoralis DSM 5473, the separate ORFs fuse into a single ORF, and the putative amino acid sequence of is homologous to that of the Thermococcus sibiricus TSIB_0634 gene. Although there is a possibility that this OCD-like protein from Thermococcus litoralis DSM 5473 forms a heterooligomeric structure, the TlLhpI gene is synthesized as a single ORF by sequential steps of PCR using sense and antisense primers and genome DNA of Thermococcus litoralis DSM 5473 as a template
Thermococcus litoralis
V224D/A228K
12fold enhancement of DELTA1-pyrroline-2-carboxylate reductase activity, 6.7fold enhancement for the oxidization activity for L-proline, no alanine dehydrogenase activity
Thermococcus litoralis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.444
-
L-proline
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis
0.608
-
L-pipecolate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
0.944
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
1.12
-
L-proline
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
2.77
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Thermococcus litoralis
72000
-
gel filtration
Thermococcus litoralis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
DELTA1-pyrroline-2-carboxylate + NADH + H+
Thermococcus litoralis
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
L-proline + NAD+
-
-
?
DELTA1-pyrroline-2-carboxylate + NADH + H+
Thermococcus litoralis DSM 5473
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
L-proline + NAD+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thermococcus litoralis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
cis-4-hydroxy-L-proline + NAD+
activity is 85% compared to the activity with L-proline
729750
Thermococcus litoralis
?
-
-
-
?
cis-4-hydroxy-L-proline + NAD+
activity is 85% compared to the activity with L-proline
729750
Thermococcus litoralis DSM 5473
?
-
-
-
?
DELTA1-pyrroline-2-carboxylate + NADH + H+
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
729750
Thermococcus litoralis
L-proline + NAD+
-
-
-
?
DELTA1-pyrroline-2-carboxylate + NADH + H+
preference of the reaction equilibrium in the direction toward NADH-dependent reduction
729750
Thermococcus litoralis
L-proline + NAD+
-
-
-
r
DELTA1-pyrroline-2-carboxylate + NADH + H+
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
729750
Thermococcus litoralis DSM 5473
L-proline + NAD+
-
-
-
?
DELTA1-pyrroline-2-carboxylate + NADH + H+
preference of the reaction equilibrium in the direction toward NADH-dependent reduction
729750
Thermococcus litoralis DSM 5473
L-proline + NAD+
-
-
-
r
L-pipecolate + NAD+
activity is 49% compared to the activity with L-proline
729750
Thermococcus litoralis
DELTA1-piperideine-2-carboxylate + NADH + H+
-
-
-
?
L-pipecolate + NAD+
activity is 49% compared to the activity with L-proline
729750
Thermococcus litoralis DSM 5473
DELTA1-piperideine-2-carboxylate + NADH + H+
-
-
-
?
L-proline + NAD+
preference of the reaction equilibrium in the direction toward NADH-dependent reduction
729750
Thermococcus litoralis
DELTA1-pyrroline-2-carboxylate + NADH + H+
-
-
-
r
additional information
the enzyme also shows alanine dehydrogenase activity (EC 1.4.1.1) and N-methyl-L-alanine dehydrogenase activity (EC 1.4.1.17)
729750
Thermococcus litoralis
?
-
-
-
-
additional information
the enzyme also shows alanine dehydrogenase activity (EC 1.4.1.1) and N-methyl-L-alanine dehydrogenase activity (EC 1.4.1.17)
729750
Thermococcus litoralis DSM 5473
?
-
-
-
-
trans-4-hydroxy-L-proline + NAD+
activity is 70% compared to the activity with L-proline
729750
Thermococcus litoralis
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE
Thermococcus litoralis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
assay at
Thermococcus litoralis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.2
-
L-pipecolate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
0.23
-
L-proline
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
0.61
-
L-proline
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis
16.6
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
558
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4
6
substrates: DELTA1-pyrroline-2-carboxylate + NADH + H+
Thermococcus litoralis
11
-
substrates: L-proline + NAD+
Thermococcus litoralis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
11
pH 5.0: about 40% of maximal activity, pH 11.0: about 80% of maximal activity
Thermococcus litoralis
10
12
pH 10.0: about 75% of maximal activity, pH 12.0: about 85% of maximal activity
Thermococcus litoralis
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
Thermococcus litoralis
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
Thermococcus litoralis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.2
-
L-proline
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
0.33
-
L-pipecolate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
1.4
-
L-proline
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis
17.6
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
201
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.2
-
L-proline
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
0.33
-
L-pipecolate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
1.4
-
L-proline
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis
17.6
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
201
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis
Other publictions for EC 1.5.1.B5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729750
Watanabe
Ornithine cyclodeaminase/mu-cr ...
Thermococcus litoralis, Thermococcus litoralis DSM 5473
FEBS Open Bio
4
617-626
2014
-
-
1
-
4
-
-
5
-
-
2
2
-
8
-
-
1
2
-
-
-
-
12
1
3
1
-
-
5
2
2
-
2
-
-
-
-
-
1
2
-
4
-
-
-
-
5
-
-
2
2
-
-
-
1
-
-
-
-
12
1
1
-
-
5
2
2
-
-
-
1
1
-
5
5
742532
Watanabe
Ornithine cyclodeaminase/micr ...
Thermococcus litoralis, Thermococcus litoralis DSM 5473
FEBS open bio
4
617-626
2014
-
-
1
-
3
-
-
2
-
-
-
2
-
10
-
-
1
-
-
-
2
-
11
-
3
1
-
-
2
2
-
-
2
-
-
-
-
-
1
2
-
3
-
-
-
-
2
-
-
-
2
-
-
-
1
-
-
2
-
11
-
1
-
-
2
2
-
-
-
-
1
1
-
2
2