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Literature summary for 1.5.1.9 extracted from

  • Serrano, G.C.; Rezende e Silva Figueira, T.; Kiyota, E.; Zanata, N.; Arruda, P.
    Lysine degradation through the saccharopine pathway in bacteria: LKR and SDH in bacteria and its relationship to the plant and animal enzymes (2012), FEBS Lett., 586, 905-911.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Nostoc punctiforme
expressed in Escherichia coli BL21(DE3) cells Ruegeria pomeroyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
x * 44000, calculated from amino acid sequence Nostoc punctiforme
44000
-
x * 44000, calculated from amino acid sequence Ruegeria pomeroyi
44100
-
x * 44100, SDS-PAGE Nostoc punctiforme
44100
-
x * 44100, SDS-PAGE Ruegeria pomeroyi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
saccharopine + NAD+ + H2O Nostoc punctiforme
-
L-glutamate + alpha-aminoadipate semialdehyde + NADH + H+
-
r
saccharopine + NAD+ + H2O Ruegeria pomeroyi
-
L-glutamate + alpha-aminoadipate semialdehyde + NADH + H+
-
r
saccharopine + NAD+ + H2O Nostoc punctiforme ATCC 29133
-
L-glutamate + alpha-aminoadipate semialdehyde + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Nostoc punctiforme
-
-
-
Nostoc punctiforme ATCC 29133
-
-
-
Ruegeria pomeroyi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, Ni-NTA agarose column chromatography, and Superdex 200 gel filtration Nostoc punctiforme
Q-Sepharose column chromatography, Ni-NTA agarose column chromatography, and Superdex 200 gel filtration Ruegeria pomeroyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
saccharopine + NAD+ + H2O
-
Nostoc punctiforme L-glutamate + alpha-aminoadipate semialdehyde + NADH + H+
-
r
saccharopine + NAD+ + H2O
-
Ruegeria pomeroyi L-glutamate + alpha-aminoadipate semialdehyde + NADH + H+
-
r
saccharopine + NAD+ + H2O
-
Nostoc punctiforme ATCC 29133 L-glutamate + alpha-aminoadipate semialdehyde + NADH + H+
-
r

Subunits

Subunits Comment Organism
? x * 44000, calculated from amino acid sequence Nostoc punctiforme
? x * 44000, calculated from amino acid sequence Ruegeria pomeroyi
? x * 44100, SDS-PAGE Nostoc punctiforme
? x * 44100, SDS-PAGE Ruegeria pomeroyi

Synonyms

Synonyms Comment Organism
saccharopine dehydrogenase
-
Nostoc punctiforme
saccharopine dehydrogenase
-
Ruegeria pomeroyi
SDH
-
Nostoc punctiforme
SDH
-
Ruegeria pomeroyi

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme has slightly higher affinity for NAD+ compared to NADP+ Nostoc punctiforme
NAD+ the enzyme has slightly higher affinity for NAD+ compared to NADP+ Ruegeria pomeroyi
NADP+
-
Nostoc punctiforme
NADP+
-
Ruegeria pomeroyi

pI Value

Organism Comment pI Value Maximum pI Value
Nostoc punctiforme calculated from amino acid sequence
-
5
Ruegeria pomeroyi calculated from amino acid sequence
-
5