BRENDA - Enzyme Database show
show all sequences of 1.5.1.9

LKR/SDH plays important roles throughout the tick life cycle including a long starvation period

Battur, B.; Boldbaatar, D.; Umemiya-Shirafuji, R.; Liao, M.; Battsetseg, B.; Taylor, D.; Baymbaa, B.; Fujisaki, K.; PLoS ONE 4, e7136 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli and Sf9 insect cells
Haemaphysalis longicornis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
-
calculated from amino acid sequence
Haemaphysalis longicornis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Haemaphysalis longicornis
-
; bifunctional lysine-ketoglutarate reductase/saccharopine dehydrogenase, EC 1.5.8 and EC 1.5.9
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
animal
the SDH mRNA transcripts are more abundant in unfed and starved ticks than in fed and engorged ticks
Haemaphysalis longicornis
-
midgut
-
Haemaphysalis longicornis
-
additional information
expression in all developmental stages, including a long period of starvation after detachment from the host. The mRNA transcripts are more abundant in unfed and starved ticks than in fed and engorged ticks
Haemaphysalis longicornis
-
ovary
-
Haemaphysalis longicornis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
-
700907
Haemaphysalis longicornis
L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Haemaphysalis longicornis
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
10
inactive under acidic (pH 5) or alkaline (pH 10) conditions
Haemaphysalis longicornis
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Haemaphysalis longicornis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli and Sf9 insect cells
Haemaphysalis longicornis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Haemaphysalis longicornis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
-
calculated from amino acid sequence
Haemaphysalis longicornis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
animal
the SDH mRNA transcripts are more abundant in unfed and starved ticks than in fed and engorged ticks
Haemaphysalis longicornis
-
midgut
-
Haemaphysalis longicornis
-
additional information
expression in all developmental stages, including a long period of starvation after detachment from the host. The mRNA transcripts are more abundant in unfed and starved ticks than in fed and engorged ticks
Haemaphysalis longicornis
-
ovary
-
Haemaphysalis longicornis
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
-
700907
Haemaphysalis longicornis
L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Haemaphysalis longicornis
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
10
inactive under acidic (pH 5) or alkaline (pH 10) conditions
Haemaphysalis longicornis
General Information
General Information
Commentary
Organism
physiological function
gene silencing by RNAi indicates that the tick LKR/SDH plays an integral role in the osmotic regulation of water balance and development of eggs in ovary of engorged females
Haemaphysalis longicornis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
gene silencing by RNAi indicates that the tick LKR/SDH plays an integral role in the osmotic regulation of water balance and development of eggs in ovary of engorged females
Haemaphysalis longicornis
Other publictions for EC 1.5.1.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743526
Schmidt
Lysine metabolism in antisens ...
Hordeum vulgare
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73-83
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1
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743575
Wan
Pathways of amino acid degrad ...
Nilaparvata lugens
PLoS ONE
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2015
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700907
Battur
LKR/SDH plays important roles ...
Haemaphysalis longicornis
PLoS ONE
4
e7136
2009
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1
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1
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7
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4
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1
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1
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1
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1
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694615
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Supply of nitrogen can reverse ...
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2
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2
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2
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1
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2
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2
1
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2
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1
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654174
Cunha Lima
Isolation of the bifunctional ...
Phaseolus vulgaris
Amino Acids
24
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2003
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1
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1
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657056
Stepansky
Synthesis of the Arabidopsis b ...
Arabidopsis sp.
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2
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396550
Zhu
Characterization of the two sa ...
Arabidopsis thaliana
Plant Physiol.
124
1363-1371
2000
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1
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8
1
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2
1
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1
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2
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1
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1
1
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8
1
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1
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1
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2
1
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2
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392370
Gaziola
The enzymology of lysine catab ...
Oryza sativa
Eur. J. Biochem.
247
364-371
1997
-
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2
2
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3
1
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1
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1
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2
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3
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1
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1
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2
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2
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3
1
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1
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2
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3
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392371
Miron
-
In vitro dephosphorylation inh ...
Glycine max
Plant J.
12
1453-1458
1997
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1
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2
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1
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1
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2
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2
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396547
Goncalves-Butruille
Purification and Characterizat ...
Zea mays
Plant Physiol.
110
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1996
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2
1
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1
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2
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1
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2
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1
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1
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1
2
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2
1
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1
1
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392366
Blemings
Lysine-alpha-ketoglutarate red ...
Rattus norvegicus
J. Nutr.
124
1215-1221
1994
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1
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1
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1
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396544
Mukhopadhyay
Comparison of lysine and trypt ...
Bos taurus, Rattus norvegicus
Experientia
46
874-876
1990
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4
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392365
Markovitz
The bifunctional aminoadipic s ...
Bos taurus
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1987
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1
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Markovitz
Familial hyperlysinemias. Puri ...
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2
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4
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2
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2
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2
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392361
Hutzler
Lysine-ketoglutarate reductase ...
Homo sapiens
Biochim. Biophys. Acta
377
42-51
1975
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1
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2
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3
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1
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1
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3
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2
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396540
Fjellstedt
Properties of partially purifi ...
Homo sapiens
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171
191-196
1975
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4
2
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1
1
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1
1
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1
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2
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2
1
1
2
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2
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4
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2
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1
1
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1
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1
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2
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2
1
1
-
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-
-
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392363
Fellows
Lysine metabolism in mammals ...
Bos taurus, Canis lupus familiaris, Felis catus, Homo sapiens, Ovis aries, Rattus norvegicus, Sus scrofa
Biochem. J.
136
329-334
1973
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-
-
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7
-
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8
-
7
-
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7
7
-
16
-
-
-
-
-
-
-
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7
-
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-
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-
7
-
-
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7
-
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8
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7
7
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16
-
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396543
Hutzler
Saccharopine cleavage by a deh ...
Homo sapiens
Biochim. Biophys. Acta
206
205-214
1970
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-
-
-
-
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4
2
-
3
-
1
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1
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1
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1
1
2
2
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1
1
2
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1
1
1
2
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2
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4
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2
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3
-
1
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1
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1
1
2
2
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1
1
2
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1
1
1
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