Activating Compound | Comment | Organism | Structure |
---|---|---|---|
abscisic acid | 0.05 mM, incubation for 12 h, significantly increased activity | Brassica napus | |
additional information | activity is enhanced with decreasing osmotic potential and LKR/SDH gene is upregulated by osmotic stress | Brassica napus | |
polyethylene glycol 6000 | enzyme activity increases in response to an upshock osmotic stress caused by polyethylene glycol 6000 solutions of decreasing osmotic potential with values ranging from -0.1 to -3 MPa | Brassica napus |
Cloned (Comment) | Organism |
---|---|
isolation of the cDNA and gene encoding bifunctional enzyme with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Brassica napus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate + NADPH | Brassica napus | lysine catabolism | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
L-lysine + 2-oxoglutarate + NADPH | Brassica napus | L-lysine catabolism is reversibly osmo-regulated at the level of LKR/SDH bifunctional enzyme | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica napus | - |
var. oleifera cv. Samourai | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Brassica napus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Brassica napus | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
L-lysine + 2-oxoglutarate + NADPH | lysine catabolism | Brassica napus | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
L-lysine + 2-oxoglutarate + NADPH | L-lysine catabolism is reversibly osmo-regulated at the level of LKR/SDH bifunctional enzyme | Brassica napus | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Brassica napus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | NADH can not replace NADPH | Brassica napus | |
NADPH | - |
Brassica napus |