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Literature summary for 1.5.1.8 extracted from

  • Zhu, X.; Tang, G.; Galili, G.
    The catabolic function of the alpha-aminoadipic acid pathway in plants is associated with unidirectional activity of lysine-oxoglutarate reductase, but not saccharopine dehydrogenase (2000), Biochem. J., 351, 215-220.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.1.8

Cloned(Commentary)

Cloned (Comment) Organism
bifunctional LKR/SDH cDNA and monofunctional LKR cDNA are cloned, expression in Saccharomyces cerevisiae mutant lys1, but LKR cannot complement yeast LKR null mutant because of its uni-directional catabolic activity, overexpression in wild type yeast Arabidopsis thaliana
gene lys1 encoding lysine 2-oxoglutarate reductase is cloned Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information mutant lys1 without lysine 2-oxoglutarate reductase activity Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Saccharomyces cerevisiae 5829
-
cytosol
-
 Arabidopsis thaliana 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + 2-oxoglutarate + NADPH Saccharomyces cerevisiae biosynthetic pathway of lysine in yeast and fungi N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 r
L-lysine + 2-oxoglutarate + NADPH Arabidopsis thaliana uni-directional activity plays an important role in regulating the catabolic function of alpha-aminoadipidic acid pathway in plants and stems from structure rather than its linkage to saccharopine dehydrogenase EC 1.5.1.9 N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ir
L-lysine + 2-oxoglutarate + NADPH Arabidopsis thaliana lysine catabolism N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ir
L-lysine + 2-oxoglutarate + NADPH Saccharomyces cerevisiae lysine anabolic function is regulated by complex transcriptional and post-transcriptional controls N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Saccharomyces cerevisiae
-
 strains 8973b and 8989c, mutant lys1, lacking LKR activity, used as expression system for enzyme from Arabidopsis thaliana, lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 are two separate proteins
-

Purification (Commentary)

Purification (Comment) Organism
partial purification of a recombinant bifunctional protein with lysine-2-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities and of the monofunctional lysine-2-oxoglutarate reductase domain Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine synthesis from saccharopine, reverse direction is favoured Saccharomyces cerevisiae N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O NAD+ as electron acceptor for L-lysine formation ?
L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine synthesis from saccharopine, reverse direction is favoured Saccharomyces cerevisiae N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O NAD+ as electron acceptor for L-lysine formation r
L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine synthesis from saccharopine, reverse direction is favoured Saccharomyces cerevisiae N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine synthesis from saccharopine, reverse direction is favoured Saccharomyces cerevisiae N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine r
L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Arabidopsis thaliana N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ir
L-lysine + 2-oxoglutarate + NADPH unidirectional lysine catabolic activity Arabidopsis thaliana N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ir
L-lysine + 2-oxoglutarate + NADPH biosynthetic pathway of lysine in yeast and fungi Saccharomyces cerevisiae N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 r
L-lysine + 2-oxoglutarate + NADPH uni-directional activity plays an important role in regulating the catabolic function of alpha-aminoadipidic acid pathway in plants and stems from structure rather than its linkage to saccharopine dehydrogenase EC 1.5.1.9 Arabidopsis thaliana N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ir
L-lysine + 2-oxoglutarate + NADPH lysine catabolism Arabidopsis thaliana N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ir
L-lysine + 2-oxoglutarate + NADPH lysine anabolic function is regulated by complex transcriptional and post-transcriptional controls Saccharomyces cerevisiae N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 r
additional information bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Arabidopsis thaliana ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7 His-tagged LKRp, L-lysine catabolic activity Arabidopsis thaliana
9.5
-
 L-lysine anabolic activity Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Arabidopsis thaliana