Application | Comment | Organism |
---|---|---|
medicine | autosomal recessive familial hyperlysinemia type I: combined deficiency in lysine-ketoglutarate reductase and saccharopine dehydrogenase activities, EC 1.5.1.8 and EC 1.5.1.9 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
mitochondrion | - |
Bos taurus | 5739 | - |
mitochondrion | - |
Papio sp. | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
115000 | - |
4 * 115000, single protein with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities, SDS-PAGE | Bos taurus |
115000 | - |
4 * 115000, single protein with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities, SDS-PAGE | Papio sp. |
467000 | - |
gel filtration, single protein catalyzes both lysine-ketoglutarate reductase reaction EC 1.5.1.8 and saccharopine dehydrogenase reaction EC 1.5.1.9, bifunctional enzyme: aminoadipic semialdehyde synthase | Papio sp. |
468000 | - |
gel filtration, single protein catalyzes both lysine-ketoglutarate reductase reaction EC 1.5.1.8 and saccharopine dehydrogenase reaction EC 1.5.1.9, bifunctional enzyme: aminoadipic semialdehyde synthase | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Homo sapiens | - |
- |
- |
Papio sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
500fold purification of a single protein with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Papio sp. |
single protein with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities: aminoadipic semialdehyde synthase | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
liver | - |
Bos taurus | - |
liver | - |
Papio sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17 | - |
- |
Bos taurus |
17.1 | - |
- |
Papio sp. |
Storage Stability | Organism |
---|---|
-70°C, 6 months, stable | Papio sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Homo sapiens | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Bos taurus | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Papio sp. | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
additional information | bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Homo sapiens | ? | - |
? | |
additional information | bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Bos taurus | ? | - |
? | |
additional information | bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Papio sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 115000, single protein with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities, SDS-PAGE | Bos taurus |
homotetramer | 4 * 115000, single protein with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities, SDS-PAGE | Papio sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Bos taurus |
37 | - |
assay at | Papio sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens | |
NADPH | - |
Bos taurus | |
NADPH | - |
Papio sp. |