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Literature summary for 1.5.1.8 extracted from
- Assay for lysine-ketoglutarate reductase by reversed-phase high-performance liquid chromatography (1989), J. Chromatogr., 497, 263-267.
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-lysine + 2-oxoglutarate + NADPH | Rattus norvegicus | saccharopine pathway is the major route of lysine breakdown | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
r |  |
| L-lysine + 2-oxoglutarate + NADPH | Rattus norvegicus | lysine catabolism | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
pregnant Sprague-Dawley rats | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
HPLC lysine-ketoglutarate reductase assay | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Rattus norvegicus | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | r | |
| L-lysine + 2-oxoglutarate + NADPH | saccharopine pathway is the major route of lysine breakdown | Rattus norvegicus | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
r | |
| L-lysine + 2-oxoglutarate + NADPH | lysine catabolism | Rattus norvegicus | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Rattus norvegicus | |
| NADPH | - |
Rattus norvegicus | |
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