Literature summary for 1.5.1.6 extracted from

Strickland, K.C.; Krupenko, N.I.; Dubard, M.E.; Hu, C.J.; Tsybovsky, Y.; Krupenko, S.A.
Enzymatic properties of ALDH1L2, a mitochondrial 10-formyltetrahydrofolate dehydrogenase (2011), Chem. Biol. Interact., 191, 129-136.

Search for more literature for 1.5.1.6

Activating Compound

Activating Compound	Comment	Organism	Structure
4'-phosphopantetheinyl transferase	4'-phosphopantetheinyl transferase activates dehydrogenase catalysis of ALDH1L2 by modifying Ser375	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) Codon Plus cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
S374A	the mutant is activated by 4'-phosphopantetheinyl transferase	Homo sapiens
S374A/S375A	the mutant is not activated by 4'-phosphopantetheinyl transferase	Homo sapiens
S375A	the mutant is not activated by 4'-phosphopantetheinyl transferase	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0114	-	10-formyltetrahydrofolate	at pH 7.0 and 30°C	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Homo sapiens	5739	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
14500	-	4 * 14500, C-terminal domain, SDS-PAGE	Homo sapiens
58000	-	C-terminal domain, gel filtration	Homo sapiens
100000	-	full-length enzyme, gel filtration	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
10-formyltetrahydrofolate + NADP+ + H2O	Homo sapiens	-	tetrahydrofolate + CO2 + NADPH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
additional information	S375 is the putative site of enzyme phosphopantetheinylation	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
10-formyltetrahydrofolate + NADP+ + H2O	-	Homo sapiens	tetrahydrofolate + CO2 + NADPH + H+	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 14500, C-terminal domain, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
10-fTHF dehydrogenase	-	Homo sapiens
ALDH1L2	-	Homo sapiens
mtFDH	mitochondrial isoform	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Homo sapiens

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Release 2023.1 (January 2023)