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Literature summary for 1.5.1.6 extracted from

  • Donato, H.; Krupenko, N.I.; Tsybovsky, Y.; Krupenko, S.A.
    10-formyltetrahydrofolate dehydrogenase requires a 4-phosphopantetheine prosthetic group for catalysis (2007), J. Biol. Chem., 282, 34159-34166.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli and Sf9 insect cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
S354A no dehydrogenase activity Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information FDH requires posttranslational modification to become a functional enzyme (4'-phosphopantetheine prosthetic group) Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
5-fTHFSepharose column chromatography, Sephacryl S-300 gel filtration, and MonoQ column chromatography Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
10-formyltetrahydrofolate + NADP+ + H2O = tetrahydrofolate + CO2 + NADPH + H+ FDH catalyzes the hydrolytic cleavage of the formyl group in the N-terminal catalytic domain, followed by NADP+-dependent oxidation of the formyl group toCO2 in the C-terminal aldehyde dehydrogenase domain Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + NADP+ + H2O
-
Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
10-formyltetrahydrofolate dehydrogenase
-
Rattus norvegicus
ALDH1L1
-
Rattus norvegicus
FDH the enzyme has also hydrolase and aldehyde dehydrogenase activities Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADP+ dependent Rattus norvegicus