Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli and Sf9 insect cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
S354A | no dehydrogenase activity | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | FDH requires posttranslational modification to become a functional enzyme (4'-phosphopantetheine prosthetic group) | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
5-fTHFSepharose column chromatography, Sephacryl S-300 gel filtration, and MonoQ column chromatography | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O = tetrahydrofolate + CO2 + NADPH + H+ | FDH catalyzes the hydrolytic cleavage of the formyl group in the N-terminal catalytic domain, followed by NADP+-dependent oxidation of the formyl group toCO2 in the C-terminal aldehyde dehydrogenase domain | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O | - |
Rattus norvegicus | tetrahydrofolate + CO2 + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
10-formyltetrahydrofolate dehydrogenase | - |
Rattus norvegicus |
ALDH1L1 | - |
Rattus norvegicus |
FDH | the enzyme has also hydrolase and aldehyde dehydrogenase activities | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | dependent | Rattus norvegicus |