BRENDA - Enzyme Database
show all sequences of 1.5.1.6

The crystal structure of the hydrolase domain of 10-formyltetrahydrofolate dehydrogenase: mechanism of hydrolysis and its interplay with the dehydrogenase domain

Chumanevich, A.A.; Krupenko, S.A.; Davies, C.; J. Biol. Chem. 279, 14355-14364 (2004)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization (Commentary)
Organism
crystal structure of the hydrolase domain of FDH, solved to 2.3 A resolution
Rattus norvegicus
Engineering
Protein Variants
Commentary
Organism
C152A
95% of the activity with wild-type enzyme
Rattus norvegicus
C17A
94% of the activity with wild-type enzyme
Rattus norvegicus
C191A
96% of the activity with wild-type enzyme
Rattus norvegicus
C238A
102% of the activity with wild-type enzyme
Rattus norvegicus
C86A
110% of the activity with wild-type enzyme
Rattus norvegicus
Organism
Organism
UniProt
Commentary
Textmining
Rattus norvegicus
P28037
-
-
Synonyms
Synonyms
Commentary
Organism
10-formyltetrahydrofolate dehydrogenase
-
Rattus norvegicus
FDH
-
Rattus norvegicus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
crystal structure of the hydrolase domain of FDH, solved to 2.3 A resolution
Rattus norvegicus
Engineering (protein specific)
Protein Variants
Commentary
Organism
C152A
95% of the activity with wild-type enzyme
Rattus norvegicus
C17A
94% of the activity with wild-type enzyme
Rattus norvegicus
C191A
96% of the activity with wild-type enzyme
Rattus norvegicus
C238A
102% of the activity with wild-type enzyme
Rattus norvegicus
C86A
110% of the activity with wild-type enzyme
Rattus norvegicus
Other publictions for EC 1.5.1.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742257
Tsybovsky
The mechanism of discriminati ...
Rattus norvegicus
Chem. Biol. Interact.
202
62-69
2013
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724712
Strickland
Enzymatic properties of ALDH1L ...
Homo sapiens
Chem. Biol. Interact.
191
129-136
2011
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712429
Strickland
Acyl carrier protein-specific ...
Homo sapiens
J. Biol. Chem.
285
1627-1633
2010
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1
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712450
Krupenko
ALDH1L2 is the mitochondrial h ...
Homo sapiens
J. Biol. Chem.
285
23056-23063
2010
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2
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2
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9
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9
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1
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713460
Chang
Zebrafish 10-formyltetrahydrof ...
Danio rerio
Protein Expr. Purif.
72
217-222
2010
-
-
1
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2
-
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2
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-
12
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1
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1
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1
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1
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1
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1
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713003
Ghose
10-formyltetrahydrofolate dehy ...
Homo sapiens
Mol. Cancer Res.
7
99-107
2009
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1
1
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697029
McNeil
Disruption of lipid metabolism ...
Rattus norvegicus
Br. J. Nutr.
99
262-271
2008
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1
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2
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674389
Kim
Rat liver 10-formyltetrahydrof ...
Rattus norvegicus
J. Biochem. Mol. Biol.
40
604-609
2007
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7
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685138
Tsybovsky
Crystal structures of the carb ...
Rattus norvegicus
Biochemistry
46
2917-2929
2007
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1
1
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4
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1
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1
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1
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687640
Donato
10-formyltetrahydrofolate dehy ...
Rattus norvegicus
J. Biol. Chem.
282
34159-34166
2007
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1
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1
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1
1
1
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671194
Min
Effects of chronic ethanol ing ...
Rattus norvegicus
Alcohol. Clin. Exp. Res.
29
2188-2193
2005
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1
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4
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3
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671840
Oleinik
Cancer cells activate p53 in r ...
Homo sapiens
Biochem. J.
391
503-511
2005
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1
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1
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652526
Chumanevich
The crystal structure of the h ...
Rattus norvegicus
J. Biol. Chem.
279
14355-14364
2004
-
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1
5
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656137
Reuland
Disruption of a calmodulin cen ...
Rattus norvegicus
J. Biol. Chem.
278
22894-22900
2003
-
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9
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4
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1
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2
1
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2
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9
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1
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2
1
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656770
Oleinik
Ectopic expression of 10-formy ...
Homo sapiens
Mol. Cancer Res.
1
577-588
2003
-
-
-
-
-
-
-
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-
-
-
1
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1
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1
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2
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2
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1
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1
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1
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1
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2
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-
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-
-
-
392350
Krupenko
10-Formyltetrahydrofolate dehy ...
Homo sapiens, Rattus norvegicus
Cell Growth Differ.
13
227-236
2002
-
-
2
-
2
-
-
-
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-
-
2
-
4
-
-
-
-
-
23
-
-
3
-
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-
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-
-
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-
6
-
-
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-
-
2
6
-
2
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-
2
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-
23
-
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3
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-
-
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392346
Krupenko
On the role of conserved histi ...
Rattus norvegicus
J. Biol. Chem.
276
24030-24037
2001
-
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1
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2
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1
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1
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1
1
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1
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2
1
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1
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3
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1
3
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2
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1
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1
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1
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-
2
1
1
-
-
-
-
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-
-
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392345
Krupenko
Aspartate 142 is involved in b ...
Rattus norvegicus
J. Biol. Chem.
274
35777-35784
1999
-
-
1
-
1
-
-
-
1
-
-
1
-
1
-
-
1
-
-
1
-
-
5
1
-
1
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-
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3
-
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-
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1
3
-
1
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1
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1
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1
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1
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5
1
1
-
-
-
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-
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392348
Hong
Isolation and characterization ...
Homo sapiens
Biochem. Mol. Biol. Int.
47
407-415
1999
-
1
1
-
-
-
-
-
1
-
1
1
-
9
-
1
-
-
-
8
-
-
2
1
-
-
-
-
-
-
-
-
3
-
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1
1
3
-
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-
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1
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1
1
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1
-
-
8
-
-
2
1
-
-
-
-
-
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-
-
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-
-
-
-
392347
Krupenko
Overexpression of functional h ...
Rattus norvegicus
Protein Expr. Purif.
14
146-152
1998
-
-
1
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-
-
-
-
1
-
1
-
-
11
-
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1
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7
-
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2
2
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3
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1
3
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1
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1
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1
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7
-
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2
2
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-
392352
Kim
Identification of protein-argi ...
Rattus norvegicus
J. Biol. Chem.
273
27374-27382
1998
1
-
1
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-
1
-
1
-
2
1
-
4
-
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1
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1
1
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5
1
1
-
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4
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1
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1
4
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1
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1
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2
1
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1
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1
1
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5
1
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-
-
-
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-
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392349
Kim
Properties of tetrahydropteroy ...
Oryctolagus cuniculus
Biochemistry
35
15772-15783
1996
3
-
-
-
-
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2
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1
-
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2
-
4
-
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1
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-
2
1
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6
-
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1
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2
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5
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3
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5
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2
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1
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2
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1
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2
1
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6
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1
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2
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-
392355
Martinasevic
Folate and 10-formyltetrahydro ...
Homo sapiens, Rattus norvegicus
Toxicol. Appl. Pharmacol.
141
373-381
1996
-
2
-
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4
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2
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14
-
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8
-
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4
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4
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2
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4
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4
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2
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8
-
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4
-
-
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-
-
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-
-
-
392342
Cook
Enzymatic activities of rat li ...
Oryctolagus cuniculus, Rattus norvegicus
Arch. Biochem. Biophys.
321
336-344
1995
1
-
2
-
1
-
8
1
4
-
1
1
-
5
1
-
1
1
-
4
-
-
9
1
1
2
-
-
-
-
-
-
9
1
-
-
1
-
2
9
-
1
-
-
8
1
1
4
-
1
1
-
1
-
1
-
4
-
-
9
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
392343
Wagner
10-Formyltetrahydrofolate dehy ...
Rattus norvegicus, Sus scrofa
Arch. Biochem. Biophys.
316
141-147
1995
-
-
1
-
-
-
4
-
2
-
1
4
-
5
-
-
1
-
-
3
-
1
11
1
-
-
-
-
-
-
-
-
8
-
-
-
-
-
1
8
-
-
-
-
5
-
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2
-
1
4
-
-
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1
-
3
-
1
11
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392351
Krupenko
-
Recombinant 10-formyltetrahydr ...
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Cysteine 707 is involved in th ...
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Champion
Identification of a heritable ...
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Johlin
Studies on the mechanism of me ...
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Case
Resolution of rat liver 10-for ...
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392339
Min
Identification of 10-formyltet ...
Rattus norvegicus, Sus scrofa
Biochim. Biophys. Acta
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348-353
1988
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392337
Rios-Orlandi
Formyltetrahydrofolate dehydro ...
Sus scrofa
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1986
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Scrutton
Inhibitory effects of histidin ...
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392335
Kutzbach
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10-Formyl tetrahydrofolate:NAD ...
Gallus gallus, Rattus norvegicus, Sus scrofa
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1971
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Kutzbach
Partial purification of a 10-f ...
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1968
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