Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | dehydrogenase and hydrolase activities are dependent on the presence of high concentrations of 2-mercaptoethanol to prevent the oxidative degradation of the substrate 10-formyltetrahydrofolate | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
cloning of the cDNA encoding FDH | Rattus norvegicus |
expression of FDH cDNA, subcloned in the pVL 1393 baculovirus vector, in Sf9 insect cells, using the MaxBac expression system | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00086 | - |
NADP+ | with 10-formyl-5,8-dideazafolate as substrate | Rattus norvegicus | |
0.00088 | - |
NADP+ | with 10-formyltetrahydrofolate as substrate | Rattus norvegicus | |
0.0032 | - |
10-formyl-5,8-dideazafolate | - |
Rattus norvegicus | |
0.0055 | - |
10-formyltetrahydrofolate | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O | Rattus norvegicus | natural substrate | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydropteroylpentaglutamate + NADP+ + H2O | Rattus norvegicus | natural substrate | tetrahydropteroylpentaglutamate + CO2 + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.144 | - |
with 10-formyl-5,8-dideazafolate as substrate | Rattus norvegicus |
0.26 | - |
with 10-formyltetrahydrofolate as substrate | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyl-5,8-dideazafolate + NADP+ + H2O | good substrate | Rattus norvegicus | 5,8-dideazafolate + CO2 + NADPH + H+ | - |
? | |
10-formyl-5,8-dideazafolate + NADP+ + H2O | stable synthetic analogue can substitute for the labile natural substrate, affinity is twice as high as for 10-formyltetrahydrofolate | Rattus norvegicus | 5,8-dideazafolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydrofolate + NADP+ + H2O | - |
Rattus norvegicus | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydrofolate + NADP+ + H2O | natural substrate | Rattus norvegicus | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydropteroylpentaglutamate + NADP+ + H2O | natural substrate | Rattus norvegicus | tetrahydropteroylpentaglutamate + CO2 + NADPH | - |
? | |
additional information | bifunctional enzyme: NADP+-dependent dehydrogenase activity and NADP+-independent hydrolase activity of 10-formyltetrahydrofolate with two active sites | Rattus norvegicus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
10-formyltetrahydrofolate | 10-formyltetrahydrofolate | Rattus norvegicus | |
10-formyltetrahydrofolate | 10-formyltetrahydrofolate is one of the most labile of the reduced coenzymes | Rattus norvegicus | |
folate | 10-formyl-5,8-dideazafolate and 5,8-dideazafolate | Rattus norvegicus | |
NADP+ | NADP+-dependent | Rattus norvegicus | |
tetrahydrofolate | - |
Rattus norvegicus |