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Literature summary for extracted from

  • Lu, C.; Liu, Y.; Li, J.; Liu, L.; Du, G.
    Engineering of biosynthesis pathway and NADPH supply for improved L-5-methyltetrahydrofolate production by Lactococcus lactis (2021), J. Microbiol. Biotechnol., 31, 154-162 .
    View publication on PubMed


Application Comment Organism
synthesis improvement of the biosynthesis of 5-MTHF by Lactococcus lactis by systematic pathway engineering, including the overexpression of the key rate-limiting enzyme MTHFR, strengthening 5-MTHF biosynthesis and folate supply by coexpression of the drfA, metF, and folE genes, increasing NADPH supply by overexpression of G6PDH, and increasing the proportion of 5-MTHF in various folates by overexpressing 5-formyltetrahydrofolate cyclo-ligase. The 5-MTHF titer finally reaches 300 microg/l with key precursor addition Lactococcus lactis subsp. lactis


Organism UniProt Comment Textmining
Lactococcus lactis subsp. lactis A0A0B8QP67


Synonyms Comment Organism
Lactococcus lactis subsp. lactis