Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.1.54 extracted from

  • Roje, S.; Wang, H.; McNeil, S.D.; Raymond, R.K.; Appling, D.R.; Shachar-Hill, Y.; Bohnert, H.J.; Hanson, A.D.
    Isolation, characterization, and functional expression of cDNAs encoding NADH-dependent methylenetetrahydrofolate reductase from higher plants (1999), J. Biol. Chem., 274, 36089-36096 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
S-adenosyl-L-methionine stimulation by 10-20% Arabidopsis thaliana
S-adenosyl-L-methionine stimulation by 50-70% Arabidopsis thaliana

General Stability

General Stability Organism
unstable, loss of 50% of activity during 30 min on ice Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: S-Methyl-L-methionine; not inhibitory: S-Methyl-L-methionine Arabidopsis thaliana
additional information not inhibitory: S-adenosyl-L-methionine and S-Methyl-L-methionine Zea mays

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O80585 isoform MTHFR2, cf. EC 1.5.1.20
-
Arabidopsis thaliana Q9SE60 isoform MTHFR1, cf. EC 1.5.1.20
-
Zea mays Q9SE94 cf. EC 1.5.1.20
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
6.9
-
pH 7.2, 30Ā°C Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + menadiol
-
Zea mays 5-methyltetrahydrofolate + menadione
-
r
5,10-methylenetetrahydrofolate + menadiol
-
Arabidopsis thaliana 5-methyltetrahydrofolate + menadione
-
r
5,10-methylenetetrahydrofolate + NADH + H+
-
Zea mays 5-methyltetrahydrofolate + NAD+
-
?
5,10-methylenetetrahydrofolate + NADH + H+
-
Arabidopsis thaliana 5-methyltetrahydrofolate + NAD+
-
?
5-methyltetrahydrofolate + menadione
-
Zea mays 5,10-methylenetetrahydrofolate + menadiol
-
r
5-methyltetrahydrofolate + menadione
-
Arabidopsis thaliana 5,10-methylenetetrahydrofolate + menadiol
-
r

Subunits

Subunits Comment Organism
? x * 66300, calculated from sequence and SDS-PAGE Arabidopsis thaliana
? x * 66800, calculated from sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
MTHFR1
-
Arabidopsis thaliana
MTHFR2
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADH enzyme strongly prefers NADH over NADPH Zea mays
NADH enzyme strongly prefers NADH over NADPH Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function enzyme is able to complement a Saccharomyces cerevisiae met12 met13 double disruptant Zea mays
physiological function enzyme is able to complement a Saccharomyces cerevisiae met12 met13 double disruptant Arabidopsis thaliana