Activating Compound | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | stimulation by 10-20% | Arabidopsis thaliana | |
S-adenosyl-L-methionine | stimulation by 50-70% | Arabidopsis thaliana |
General Stability | Organism |
---|---|
unstable, loss of 50% of activity during 30 min on ice | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: S-Methyl-L-methionine; not inhibitory: S-Methyl-L-methionine | Arabidopsis thaliana | |
additional information | not inhibitory: S-adenosyl-L-methionine and S-Methyl-L-methionine | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O80585 | isoform MTHFR2, cf. EC 1.5.1.20 | - |
Arabidopsis thaliana | Q9SE60 | isoform MTHFR1, cf. EC 1.5.1.20 | - |
Zea mays | Q9SE94 | cf. EC 1.5.1.20 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.9 | - |
pH 7.2, 30°C | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + menadiol | - |
Zea mays | 5-methyltetrahydrofolate + menadione | - |
r | |
5,10-methylenetetrahydrofolate + menadiol | - |
Arabidopsis thaliana | 5-methyltetrahydrofolate + menadione | - |
r | |
5,10-methylenetetrahydrofolate + NADH + H+ | - |
Zea mays | 5-methyltetrahydrofolate + NAD+ | - |
? | |
5,10-methylenetetrahydrofolate + NADH + H+ | - |
Arabidopsis thaliana | 5-methyltetrahydrofolate + NAD+ | - |
? | |
5-methyltetrahydrofolate + menadione | - |
Zea mays | 5,10-methylenetetrahydrofolate + menadiol | - |
r | |
5-methyltetrahydrofolate + menadione | - |
Arabidopsis thaliana | 5,10-methylenetetrahydrofolate + menadiol | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 66300, calculated from sequence and SDS-PAGE | Arabidopsis thaliana |
? | x * 66800, calculated from sequence | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
MTHFR1 | - |
Arabidopsis thaliana |
MTHFR2 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | enzyme strongly prefers NADH over NADPH | Zea mays | |
NADH | enzyme strongly prefers NADH over NADPH | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme is able to complement a Saccharomyces cerevisiae met12 met13 double disruptant | Zea mays |
physiological function | enzyme is able to complement a Saccharomyces cerevisiae met12 met13 double disruptant | Arabidopsis thaliana |