Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.1.54 extracted from

  • Bertsch, J.; Oeppinger, C.; Hess, V.; Langer, J.; Mueller, V.
    Heterotrimeric NADH-oxidizing methylenetetrahydrofolate reductase from the acetogenic bacterium Acetobacterium woodii (2015), J. Bacteriol., 197, 1681-1689 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information the NADH:methylenetetrahydrofolate reductase activity is not stimulated by ferredoxin Acetobacterium woodii

Metals/Ions

Metals/Ions Comment Organism Structure
Fe complex contains 23.5 Fe and 24.5 S, corresponding to predicted six [4Fe4S] clusters in MetV and RnfC2 Acetobacterium woodii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
PAGE Acetobacterium woodii

Organism

Organism UniProt Comment Textmining
Acetobacterium woodii H6LBX9 cf. EC 1.5.1.20
-
Acetobacterium woodii DSM 1030 H6LBX9 cf. EC 1.5.1.20
-

Purification (Commentary)

Purification (Comment) Organism
-
Acetobacterium woodii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
248
-
substrate 5,10-methylenetetrahydrofolate, pH 7.0, 30Ā°C Acetobacterium woodii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + NADH + H+
-
Acetobacterium woodii 5-methyltetrahydrofolate + NAD+
-
ir
5,10-methylenetetrahydrofolate + NADH + H+
-
Acetobacterium woodii DSM 1030 5-methyltetrahydrofolate + NAD+
-
ir
additional information no substrate: ferredoxin Acetobacterium woodii ?
-
-
additional information no substrate: ferredoxin Acetobacterium woodii DSM 1030 ?
-
-
NADH + H+ + ferricyanide
-
Acetobacterium woodii NAD+ + ferrocyanide
-
r
NADH + H+ + ferricyanide
-
Acetobacterium woodii DSM 1030 NAD+ + ferrocyanide
-
r
NADH + H+ + methylviologen
-
Acetobacterium woodii NAD+ + reduced methylviologen
-
r
NADH + H+ + methylviologen
-
Acetobacterium woodii DSM 1030 NAD+ + reduced methylviologen
-
r

Subunits

Subunits Comment Organism
heterotrimer 1 * 71000, i.e.subunit RnfC2, 1 * 32800, i.e. subunit MetF, 1 * 22700, i.e. subunit MetV, respectively Acetobacterium woodii

Synonyms

Synonyms Comment Organism
metF
-
Acetobacterium woodii

Cofactor

Cofactor Comment Organism Structure
FMN complex contains 2 flavin mononucleotides Acetobacterium woodii
[4Fe-4S]-center complex contains 23.5 Fe and 24.5 S, corresponding to predicted six [4Fe4S] clusters in MetV and RnfC2 Acetobacterium woodii