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Literature summary for 1.5.1.54 extracted from

  • Sheppard, C.A.; Trimmer, E.E.; Matthews, R.G.
    Purification and properties of NADH-dependent 5, 10-methylenetetrahydrofolate reductase (MetF) from Escherichia coli (1999), J. Bacteriol., 181, 718-725 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
5,10-methylenetetrahydrofolate substrate inhibition Escherichia coli
NADH substrate inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
5,10-methylenetetrahydrofolate pH 7.2, 15┬░C Escherichia coli
0.013
-
NADH pH 7.2, 15┬░C Escherichia coli
0.075
-
5-methyltetrahydrofolate pH 7.2, 15┬░C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
119000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AEZ1 cf. EC 1.5.1.20
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Escherichia coli

Storage Stability

Storage Stability Organism
-80┬░C, 50 mM phosphate buffer, pH 7.2, 10% glycerol, 0.3 mM EDTA, stable for up to one year Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + menadiol
-
Escherichia coli 5-methyltetrahydrofolate + menadione
-
r
5,10-methylenetetrahydrofolate + NADH + H+
-
Escherichia coli 5-methyltetrahydrofolate + NAD+
-
?
5-methyltetrahydrofolate + menadione
-
Escherichia coli 5,10-methylenetetrahydrofolate + menadiol
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 33100, calculated from sequence Escherichia coli

Synonyms

Synonyms Comment Organism
metF
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
30
-
NADH pH 7.2, 15┬░C Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FAD 1 FAD per subunit Escherichia coli
additional information no cofactor: NADPH Escherichia coli
NADH
-
Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0071
-
5,10-methylenetetrahydrofolate pH 7.2, 15┬░C Escherichia coli
0.009
-
NADH pH 7.2, 15┬░C Escherichia coli