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Literature summary for 1.5.1.54 extracted from

  • Wohlfarth, G.; Geerligs, G.; Diekert, G.
    Purification and properties of a NADH-dependent 5,10-methylenetetrahydrofolate reductase from Peptostreptococcus productus (1990), Eur. J. Biochem., 192, 411-417 .
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
NADH pH 5.5, 37┬░C Blautia producta

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane associated to membrane Blautia producta 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
250000
-
gel filtration Blautia producta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + NADH + H+ Blautia producta
-
5-methyltetrahydrofolate + NAD+
-
?
5,10-methylenetetrahydrofolate + NADH + H+ Blautia producta Marburg
-
5-methyltetrahydrofolate + NAD+
-
?

Organism

Organism UniProt Comment Textmining
Blautia producta
-
-
-
Blautia producta Marburg
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Blautia producta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
380
-
pH 5.5, 37┬░C Blautia producta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + NADH + H+
-
Blautia producta 5-methyltetrahydrofolate + NAD+
-
?
5,10-methylenetetrahydrofolate + NADH + H+
-
Blautia producta Marburg 5-methyltetrahydrofolate + NAD+
-
?
5,10-methylenetetrahydrofolate + reduced methylene blue
-
Blautia producta 5-methyltetrahydrofolate + methylene blue
-
r
5,10-methylenetetrahydrofolate + reduced methylene blue
-
Blautia producta Marburg 5-methyltetrahydrofolate + methylene blue
-
r
5-methyltetrahydrofolate + methylene blue
-
Blautia producta 5,10-methylenetetrahydrofolate + reduced methylene blue
-
r
5-methyltetrahydrofolate + methylene blue
-
Blautia producta Marburg 5,10-methylenetetrahydrofolate + reduced methylene blue
-
r
additional information the enzyme catalyzes the methylenetetrahydrofolate reduction with methylene blue as an artificial electron donor. The oxidation of methyltetrahydrofolate is mediated with methylene blue as the electron acceptor. Neither NAD+ nor viologen dyes can replace methylene blue in this reaction Blautia producta ?
-
-
additional information the enzyme catalyzes the methylenetetrahydrofolate reduction with methylene blue as an artificial electron donor. The oxidation of methyltetrahydrofolate is mediated with methylene blue as the electron acceptor. Neither NAD+ nor viologen dyes can replace methylene blue in this reaction Blautia producta Marburg ?
-
-

Subunits

Subunits Comment Organism
octamer 8 * 32000, SDS-PAGE Blautia producta

Cofactor

Cofactor Comment Organism Structure
FAD 4 FAD/mol octamer Blautia producta
additional information no cosubstrates: NADPH, FAD(H2) Blautia producta
NADH
-
Blautia producta