Cloned (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | chromosomal copy of MET13 is replaced by an Arabidopsis thaliana MTHFR cDNA (AtMTHFR-1) or by a chimeric sequence (Chimera-1) comprising the yeast N-terminal domain and the AtMTHFR-1 C-terminal domain. Chimera-1 uses both NADH and NADPH and is insensitive to S-adenosyl-L-methionine. Engineered yeast expressing Chimera-1 accumulates 140fold more S-adenosyl-L-methionine and 7fold more methionine than does the wild-type and grows normally. Yeast expressing AtMTHFR-1 accumulates 8fold more S-adenosyl-L-methionine | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
5,10-methylenetetrahydrofolate | wild-type, cosubstrate NADPH, pH 7.2, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.011 | - |
NADPH | wild-type, pH 7.2, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.106 | - |
5,10-methylenetetrahydrofolate | Chimera-1, cosubstrate NADPH, pH 7.2, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.152 | - |
5,10-methylenetetrahydrofolate | Chimera-1, cosubstrate NADH, pH 7.2, temperature not specified in the publication | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P53128 | cf. EC 1.5.1.20 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NADH + H+ | - |
Saccharomyces cerevisiae | 5-methyltetrahydrofolate + NAD+ | - |
? | |
5,10-methylenetetrahydrofolate + NADPH + H+ | - |
Saccharomyces cerevisiae | 5-methyltetrahydrofolate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MET13 | - |
Saccharomyces cerevisiae |
MTHFR | - |
Saccharomyces cerevisiae |