Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | strong inhibition | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
5,10-methylenetetrahydrofolate | pH 7.2, 30°C | Sus scrofa | |
0.033 | - |
NADPH | pH 7.2, 30°C | Sus scrofa | |
0.31 | - |
NADH | pH 7.2, 30°C | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NADPH + H+ | Sus scrofa | - |
5-methyltetrahydrofolate + NADP+ | - |
? | |
additional information | Sus scrofa | enzyme is specific for the (+) diastereoisomer of 5,10-methylenetetrahydrofolate as its physiological substrate. In addition, the enzyme exhibits a strong NADPH-specific diaphorase (reduced-NAD:lipoamide oxidoreductase, EC 1.8.1.4) or menadione reductase (reduced-NAD(P):(acceptor) oxidoreductase, EC 1.6.5.2 ) activity | ? | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NADH + H+ | NADH is a poor cofactor | Sus scrofa | 5-methyltetrahydrofolate + NAD+ | - |
? | |
5,10-methylenetetrahydrofolate + NADPH + H+ | - |
Sus scrofa | 5-methyltetrahydrofolate + NADP+ | - |
? | |
5-methyltetrahydrofolate + oxidized menadione | - |
Sus scrofa | 5,10-methylenetetrahydrofolate + reduced menadione | - |
? | |
additional information | enzyme is specific for the (+) diastereoisomer of 5,10-methylenetetrahydrofolate as its physiological substrate. In addition, the enzyme exhibits a strong NADPH-specific diaphorase (reduced-NAD:lipoamide oxidoreductase, EC 1.8.1.4) or menadione reductase (reduced-NAD(P):(acceptor) oxidoreductase, EC 1.6.5.2 ) activity | Sus scrofa | ? | - |
- |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | NADPH is 10fold preferred over NADH | Sus scrofa | |
NADPH | enzyme is specifc for NADPH | Sus scrofa |