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Literature summary for 1.5.1.5 extracted from

  • MacKenzie, R.E.; Tan, L.U.L.
    Methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase: a multifunctional protein from porcine liver (1980), Methods Enzymol., 66, 609-615.
    View publication on PubMed

General Stability

General Stability Organism
relatively labile, stabilized with phosphate, glycerol and NADP+ Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
NADP+
-
Sus scrofa
0.022
-
5,10-methylenetetrahydrofolate
-
Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
x * 33000, fragment has methylenetetrahydrofolate dehadrogenase and methenyltetrahydrofolate cyclohydrase activity, proteolysis, SDS-PAGE Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
trifunctional enzyme: methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrase, formyltetrahydrofolate synthetase Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
61
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
0-4°C, after 1 month retains 75% of the methylenetetrahydrofolate dehydrogenase activity Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + NADP+
-
Sus scrofa 5,10-methenyltetrahydrofolate + NADPH + H+
-
?

Subunits

Subunits Comment Organism
? x * 33000, fragment has methylenetetrahydrofolate dehadrogenase and methenyltetrahydrofolate cyclohydrase activity, proteolysis, SDS-PAGE Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Sus scrofa