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Literature summary for 1.5.1.5 extracted from
- 5,10-Methylenetetrahydrofolic dehydrogenase from bakers'yeast (1962), J. Biol. Chem., 237, 1982-1988.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-Dimercaptopropanol | the most effective thiol | Saccharomyces cerevisiae |  |
| 2-mercaptoethanol | - |
Saccharomyces cerevisiae | |
| cysteine | - |
Saccharomyces cerevisiae | |
| formaldehyde | maximal activity at 0.3 mM | Saccharomyces cerevisiae | |
| glutathione | - |
Saccharomyces cerevisiae | |
General Stability
| General Stability | Organism |
|---|---|
| frozen stable for 12 days, loss of activity upon repeated freezing and thawing | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Borate | inhibition reversible by potassium | Saccharomyces cerevisiae | |
| Ca2+ | - |
Saccharomyces cerevisiae | |
| formaldehyde | 50% inhibition at 2.5 mM | Saccharomyces cerevisiae | |
| Mg2+ | - |
Saccharomyces cerevisiae | |
| p-chloromercuribenzoate | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.037 | - |
5,10-methylenetetrahydrofolate | - |
Saccharomyces cerevisiae | |
| 0.69 | - |
NADP+ | - |
Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | and other monovalent metal cations | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + NADP+ | Saccharomyces cerevisiae | - |
5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 259 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + NADP+ | - |
Saccharomyces cerevisiae | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
30 min stable, destroyed in 3 min at 60°C | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Saccharomyces cerevisiae | |
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