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Literature summary for 1.5.1.5 extracted from
- Methylenetetrahydrofolate dehydrogenase from Clostridium formicoaceticum and methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrolase (combined) from Clostridium thermoaceticum (1980), Methods Enzymol., 66, 599-609.
General Stability
| General Stability | Organism |
|---|---|
| freezing inactivates | Moorella thermoacetica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
NADP+ | - |
Moorella thermoacetica |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
2 * 30000, SDS-PAGE | Moorella thermoacetica |
| 55000 | - |
methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase combined, equilibrium ultracentrifugation | Moorella thermoacetica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + NADP+ | Moorella thermoacetica | - |
5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Moorella thermoacetica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase combined | Moorella thermoacetica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 310 | - |
assays at 37°C | Moorella thermoacetica |
| 720 | - |
at 60°C growth temperature | Moorella thermoacetica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for at least a month | Moorella thermoacetica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + NADP+ | - |
Moorella thermoacetica | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 30000, SDS-PAGE | Moorella thermoacetica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 64 | - |
above | Moorella thermoacetica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Moorella thermoacetica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Moorella thermoacetica | |
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Release 2023.1 (January 2023)
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