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Literature summary for 1.5.1.5 extracted from

  • Albrecht, A.M.; Pearce, F.K.; Hutchinson, D.J.
    Methylenetetrahydrofolate dehydrogenase of the amethopterin-resistant strain Streptococcus faecium var. durans A and its repressibility by serine (1968), J. Bacteriol., 95, 1779-1789.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.1.5

General Stability

General Stability Organism
extremely labile, during purification protection by NADP+ Enterococcus faecium

Inhibitors

Inhibitors Comment Organism Structure
guanine 48% inhibition Enterococcus faecium
Iodosobenzoate
-
 Enterococcus faecium
Mg2+ inhibition of 5% Enterococcus faecium
N-ethylmaleimide
-
 Enterococcus faecium
p-chloromercuribenzoate
-
 Enterococcus faecium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
 NADP+
-
 Enterococcus faecium
0.026
-
 5,10-methylenetetrahydrofolate
-
 Enterococcus faecium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + NADP+ Enterococcus faecium
-
 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Enterococcus faecium
-
 var.durans Ak, an amethopterin-resistent mutant
-

Purification (Commentary)

Purification (Comment) Organism
partial Enterococcus faecium

Storage Stability

Storage Stability Organism
-20°C, 0.1% bovine plasma albumin Enterococcus faecium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + NADP+
-
 Enterococcus faecium 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Enterococcus faecium

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Enterococcus faecium