BRENDA - Enzyme Database
show all sequences of 1.5.1.45

Characterization of the anthranilate degradation pathway in Geobacillus thermodenitrificans NG80-2

Liu, X.; Dong, Y.; Li, X.; Ren, Y.; Li, Y.; Wang, W.; Wang, L.; Feng, L.; Microbiology 156, 589-595 (2010)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Geobacillus thermodenitrificans
Organism
Organism
UniProt
Commentary
Textmining
Geobacillus thermodenitrificans
-
-
-
Geobacillus thermodenitrificans NG80-2
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
chelating Sepharose column chromatography
Geobacillus thermodenitrificans
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
55.47
-
using FMN and NADPH as substrates, in 20 mM glycine/NaOH (pH 9.0), at 60°C
Geobacillus thermodenitrificans
58.2
-
using FAD and NADPH as substrates, in 20 mM glycine/NaOH (pH 9.0), at 60°C
Geobacillus thermodenitrificans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
FAD + NADH + H+
95% activity with NADH relative to NADPH
709852
Geobacillus thermodenitrificans
FADH2 + NAD+
-
-
-
?
FAD + NADPH + H+
higher activity is obtained with FAD than with FMN using NADPH as the cofactor
709852
Geobacillus thermodenitrificans
FADH2 + NADP+
-
-
-
?
FMN + NADPH + H+
higher activity is obtained with FAD than with FMN using NADPH as the cofactor
709852
Geobacillus thermodenitrificans
FMNH2 + NADP+
-
-
-
?
additional information
anthranilate can also be oxidized to 3-hydroxyanthranilate by GTNG_3158 in the presence of FMN and ATP
709852
Geobacillus thermodenitrificans
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
FAD reductase
-
Geobacillus thermodenitrificans
GTNG_3158
-
Geobacillus thermodenitrificans
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Geobacillus thermodenitrificans
Purification (Commentary) (protein specific)
Commentary
Organism
chelating Sepharose column chromatography
Geobacillus thermodenitrificans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
55.47
-
using FMN and NADPH as substrates, in 20 mM glycine/NaOH (pH 9.0), at 60°C
Geobacillus thermodenitrificans
58.2
-
using FAD and NADPH as substrates, in 20 mM glycine/NaOH (pH 9.0), at 60°C
Geobacillus thermodenitrificans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
FAD + NADH + H+
95% activity with NADH relative to NADPH
709852
Geobacillus thermodenitrificans
FADH2 + NAD+
-
-
-
?
FAD + NADPH + H+
higher activity is obtained with FAD than with FMN using NADPH as the cofactor
709852
Geobacillus thermodenitrificans
FADH2 + NADP+
-
-
-
?
FMN + NADPH + H+
higher activity is obtained with FAD than with FMN using NADPH as the cofactor
709852
Geobacillus thermodenitrificans
FMNH2 + NADP+
-
-
-
?
additional information
anthranilate can also be oxidized to 3-hydroxyanthranilate by GTNG_3158 in the presence of FMN and ATP
709852
Geobacillus thermodenitrificans
?
-
-
-
-
Other publictions for EC 1.5.1.45
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
709852
Liu
Characterization of the anthra ...
Geobacillus thermodenitrificans, Geobacillus thermodenitrificans NG80-2
Microbiology
156
589-595
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
10
-
-
1
-
-
-
2
-
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-