BRENDA - Enzyme Database show
show all sequences of 1.5.1.44

In vitro conversion of chanoclavine-I aldehyde to the stereoisomers festuclavine and pyroclavine controlled by the second reduction step

Matuschek, M.; Wallwey, C.; Wollinsky, B.; Xie, X.; Li, S.; RSC Adv. 2, 3662-3669 (2012)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli XL1 Blue MRF cells
Aspergillus fumigatus
expressed in Escherichia coli XL1 Blue MRF cells
Penicillium commune
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
1 * 30000, His6-tagged enzyme, SDS-PAGe
Aspergillus fumigatus
30000
-
1 * 30000, His6-tagged enzyme, SDS-PAGe
Penicillium commune
32600
-
1 * 32600, His6-tagged enzyme, calculated from amino acid sequence
Aspergillus fumigatus
32600
-
1 * 32600, His6-tagged enzyme, calculated from amino acid sequence
Penicillium commune
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus fumigatus
-
-
-
Aspergillus fumigatus Af293
-
-
-
Claviceps purpurea
-
-
-
Penicillium commune
I6U936
-
-
Penicillium commune NRRL2033
I6U936
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA agarose column chromatography and HisPur Cobalt resin column chromatography
Aspergillus fumigatus
Ni-NTA agarose column chromatography and HisPur Cobalt resin column chromatography
Penicillium commune
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
chanoclavine-I aldehyde + NADH + H+
festuclavine synthase FgaFS and the old yellow enzyme FgaOx3 from Aspergillus fumigatus catalyse the formation of festuclavine by two reduction steps
721046
Aspergillus fumigatus
festuclavine + NAD+
-
-
-
?
chanoclavine-I aldehyde + NADH + H+
festuclavine synthase FgaFS and the old yellow enzyme FgaOx3 from Aspergillus fumigatus catalyse the formation of festuclavine by two reduction steps
721046
Penicillium commune
festuclavine + NAD+
-
-
-
?
chanoclavine-I aldehyde + NADH + H+
festuclavine synthase FgaFS and the old yellow enzyme FgaOx3 from Aspergillus fumigatus catalyse the formation of festuclavine by two reduction steps
721046
Aspergillus fumigatus Af293
festuclavine + NAD+
-
-
-
?
chanoclavine-I aldehyde + NADH + H+
festuclavine synthase FgaFS and the old yellow enzyme FgaOx3 from Aspergillus fumigatus catalyse the formation of festuclavine by two reduction steps
721046
Penicillium commune NRRL2033
festuclavine + NAD+
-
-
-
?
additional information
in the absence of reduced glutathione, agroclavine synthase EasG also converts chanoclavine-I aldehyde to pyroclavine and festuclavine in the presence of the old yellow enzyme FgaOx3 and NADPH
721046
Claviceps purpurea
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 30000, His6-tagged enzyme, SDS-PAGe; 1 * 32600, His6-tagged enzyme, calculated from amino acid sequence
Aspergillus fumigatus
monomer
1 * 30000, His6-tagged enzyme, SDS-PAGe; 1 * 32600, His6-tagged enzyme, calculated from amino acid sequence
Penicillium commune
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli XL1 Blue MRF cells
Aspergillus fumigatus
expressed in Escherichia coli XL1 Blue MRF cells
Penicillium commune
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
1 * 30000, His6-tagged enzyme, SDS-PAGe
Aspergillus fumigatus
30000
-
1 * 30000, His6-tagged enzyme, SDS-PAGe
Penicillium commune
32600
-
1 * 32600, His6-tagged enzyme, calculated from amino acid sequence
Aspergillus fumigatus
32600
-
1 * 32600, His6-tagged enzyme, calculated from amino acid sequence
Penicillium commune
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA agarose column chromatography and HisPur Cobalt resin column chromatography
Aspergillus fumigatus
Ni-NTA agarose column chromatography and HisPur Cobalt resin column chromatography
Penicillium commune
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
chanoclavine-I aldehyde + NADH + H+
festuclavine synthase FgaFS and the old yellow enzyme FgaOx3 from Aspergillus fumigatus catalyse the formation of festuclavine by two reduction steps
721046
Aspergillus fumigatus
festuclavine + NAD+
-
-
-
?
chanoclavine-I aldehyde + NADH + H+
festuclavine synthase FgaFS and the old yellow enzyme FgaOx3 from Aspergillus fumigatus catalyse the formation of festuclavine by two reduction steps
721046
Penicillium commune
festuclavine + NAD+
-
-
-
?
chanoclavine-I aldehyde + NADH + H+
festuclavine synthase FgaFS and the old yellow enzyme FgaOx3 from Aspergillus fumigatus catalyse the formation of festuclavine by two reduction steps
721046
Aspergillus fumigatus Af293
festuclavine + NAD+
-
-
-
?
chanoclavine-I aldehyde + NADH + H+
festuclavine synthase FgaFS and the old yellow enzyme FgaOx3 from Aspergillus fumigatus catalyse the formation of festuclavine by two reduction steps
721046
Penicillium commune NRRL2033
festuclavine + NAD+
-
-
-
?
additional information
in the absence of reduced glutathione, agroclavine synthase EasG also converts chanoclavine-I aldehyde to pyroclavine and festuclavine in the presence of the old yellow enzyme FgaOx3 and NADPH
721046
Claviceps purpurea
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 30000, His6-tagged enzyme, SDS-PAGe; 1 * 32600, His6-tagged enzyme, calculated from amino acid sequence
Aspergillus fumigatus
monomer
1 * 30000, His6-tagged enzyme, SDS-PAGe; 1 * 32600, His6-tagged enzyme, calculated from amino acid sequence
Penicillium commune
Other publictions for EC 1.5.1.44
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721046
Matuschek
-
In vitro conversion of chanocl ...
Aspergillus fumigatus, Aspergillus fumigatus Af293, Claviceps purpurea, Penicillium commune, Penicillium commune NRRL2033
RSC Adv.
2
3662-3669
2012
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2
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4
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14
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2
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5
2
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2
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4
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2
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5
2
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-
720361
Xie
Formyl migration product of ch ...
Aspergillus fumigatus
Magn. Reson. Chem.
49
678-681
2011
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2
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1
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1
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720617
Matuschek
New insights into ergot alkalo ...
Aspergillus fumigatus
Org. Biomol. Chem.
9
4328-4335
2011
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7
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1
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1
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719646
Cheng
Controlling a structural branc ...
Aspergillus fumigatus
J. Am. Chem. Soc.
132
12835-12837
2010
-
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2
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1
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1
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2
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1
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720615
Wallwey
Ergot alkaloid biosynthesis in ...
Aspergillus fumigatus, Aspergillus fumigatus Af293
Org. Biomol. Chem.
8
3500-3508
2010
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1
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2
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12
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1
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2
1
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1
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2
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1
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2
1
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