BRENDA - Enzyme Database show
show all sequences of 1.5.1.43

Spermidine inversely influences surface interactions and planktonic growth in Agrobacterium tumefaciens

Wang, Y.; Kim, S.H.; Natarajan, R.; Heindl, J.E.; Bruger, E.L.; Waters, C.M.; Michael, A.J.; Fuqua, C.; J. Bacteriol. 198, 2682-2691 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene Atu4170, DNA and amino acid sequence determination and analysis of wild-type enzyme mutant genes and enzymes, respectively
Agrobacterium tumefaciens
Engineering
Amino acid exchange
Commentary
Organism
additional information
transposon mutagenesis of Agrobacterium tumefaciens strain C58 to knockdown enzyme CASDH performed with the mariner minitransposon Himar1, phenotype, overview. In-frame CASDH and CASDC gene deletion mutants have severe growth defects in minimal medium but are rescued in this respect by exogenous addition of exogenous polyamines that supply the 1,3-diaminopropane group, including spermidine. The severe growth defect of the CASDH and CASDC mutants also manifested itself as a severe biofilm deficiency
Agrobacterium tumefaciens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
Agrobacterium tumefaciens
-
carboxynorspermidine + H2O + NADP+
?
-
?
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
Agrobacterium tumefaciens
-
carboxyspermidine + H2O + NADP+
?
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Agrobacterium tumefaciens
A9CG66
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
-
742792
Agrobacterium tumefaciens
carboxynorspermidine + H2O + NADP+
?
-
-
?
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
-
742792
Agrobacterium tumefaciens
carboxyspermidine + H2O + NADP+
?
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Agrobacterium tumefaciens
Cloned(Commentary) (protein specific)
Commentary
Organism
gene Atu4170, DNA and amino acid sequence determination and analysis of wild-type enzyme mutant genes and enzymes, respectively
Agrobacterium tumefaciens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Agrobacterium tumefaciens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
transposon mutagenesis of Agrobacterium tumefaciens strain C58 to knockdown enzyme CASDH performed with the mariner minitransposon Himar1, phenotype, overview. In-frame CASDH and CASDC gene deletion mutants have severe growth defects in minimal medium but are rescued in this respect by exogenous addition of exogenous polyamines that supply the 1,3-diaminopropane group, including spermidine. The severe growth defect of the CASDH and CASDC mutants also manifested itself as a severe biofilm deficiency
Agrobacterium tumefaciens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
Agrobacterium tumefaciens
-
carboxynorspermidine + H2O + NADP+
?
-
?
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
Agrobacterium tumefaciens
-
carboxyspermidine + H2O + NADP+
?
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
-
742792
Agrobacterium tumefaciens
carboxynorspermidine + H2O + NADP+
?
-
-
?
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
-
742792
Agrobacterium tumefaciens
carboxyspermidine + H2O + NADP+
?
-
-
?
General Information
General Information
Commentary
Organism
malfunction
accumulation of the precursor putrescine in the CASDH mutant The mutant accumulates homospermidine, which requires a homospermidine synthase (hss) homologue. Agrobacterium tumefaciens mutants with diminished levels of the polyamine spermidine are stimulated for biofilm formation, and exogenous provision of spermidine decreases biofilm formation. Spermidine is also essential for Agrobacterium tumefaciens growth, but the related polyamine norspermidine exogenously rescues growth and does not diminish biofilm formation, the growth requirement and biofilm control are separable. Exogenous spermidine and norspermidine restore prototrophic growth for CASDH and CASDC mutants, but only spermidine inhibits biofilm formation. CASDH and CASDC mutants accumulate homospermidine via a homospermidine synthase homologue
Agrobacterium tumefaciens
metabolism
in an alternative pathway (alternate to the pathway via S-adenosyl-L-methionine), putrescine is first converted into carboxyspermidine with the precursor L-aspartate beta-semialdehyde by the enzyme carboxyspermidine dehydrogenase (CASDH), and then carboxyspermidine is converted to spermidine by carboxyspermidine decarboxylase (CASDC). Spermidine is an essential metabolite in Agrobacterium tumefaciens and is synthesized from putrescine via the stepwise actions of carboxyspermidine dehydrogenase (CASDH) and carboxyspermidine decarboxylase (CASDC)
Agrobacterium tumefaciens
physiological function
spermidine is an essential metabolite in Agrobacterium tumefaciens and is synthesized from putrescine via the stepwise actions of carboxyspermidine dehydrogenase (CASDH) and carboxyspermidine decarboxylase (CASDC). Spermidine is essential for Agrobacterium tumefaciens growth, growth requirement and biofilm control are separable. Polyamine control of biofilm formation appears to function via effects on the cellular second messenger cyclic diguanylate monophosphate, regulating the transition from a freeliving to a surface-attached lifestyle
Agrobacterium tumefaciens
General Information (protein specific)
General Information
Commentary
Organism
malfunction
accumulation of the precursor putrescine in the CASDH mutant The mutant accumulates homospermidine, which requires a homospermidine synthase (hss) homologue. Agrobacterium tumefaciens mutants with diminished levels of the polyamine spermidine are stimulated for biofilm formation, and exogenous provision of spermidine decreases biofilm formation. Spermidine is also essential for Agrobacterium tumefaciens growth, but the related polyamine norspermidine exogenously rescues growth and does not diminish biofilm formation, the growth requirement and biofilm control are separable. Exogenous spermidine and norspermidine restore prototrophic growth for CASDH and CASDC mutants, but only spermidine inhibits biofilm formation. CASDH and CASDC mutants accumulate homospermidine via a homospermidine synthase homologue
Agrobacterium tumefaciens
metabolism
in an alternative pathway (alternate to the pathway via S-adenosyl-L-methionine), putrescine is first converted into carboxyspermidine with the precursor L-aspartate beta-semialdehyde by the enzyme carboxyspermidine dehydrogenase (CASDH), and then carboxyspermidine is converted to spermidine by carboxyspermidine decarboxylase (CASDC). Spermidine is an essential metabolite in Agrobacterium tumefaciens and is synthesized from putrescine via the stepwise actions of carboxyspermidine dehydrogenase (CASDH) and carboxyspermidine decarboxylase (CASDC)
Agrobacterium tumefaciens
physiological function
spermidine is an essential metabolite in Agrobacterium tumefaciens and is synthesized from putrescine via the stepwise actions of carboxyspermidine dehydrogenase (CASDH) and carboxyspermidine decarboxylase (CASDC). Spermidine is essential for Agrobacterium tumefaciens growth, growth requirement and biofilm control are separable. Polyamine control of biofilm formation appears to function via effects on the cellular second messenger cyclic diguanylate monophosphate, regulating the transition from a freeliving to a surface-attached lifestyle
Agrobacterium tumefaciens
Other publictions for EC 1.5.1.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741488
Kim
The essential role of spermid ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58 / ATCC 33970
ACS Chem. Biol.
11
491-499
2016
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3
3
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742792
Wang
Spermidine inversely influenc ...
Agrobacterium tumefaciens
J. Bacteriol.
198
2682-2691
2016
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3
3
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719993
Hanfrey
Alternative spermidine biosynt ...
Campylobacter jejuni
J. Biol. Chem.
286
43301-43312
2011
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704662
Shaw
Evolution and multifarious hor ...
Rhizobium leguminosarum
J. Biol. Chem.
285
14711-14723
2010
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9
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704647
Lee
An alternative polyamine biosy ...
Vibrio cholerae
J. Biol. Chem.
284
9899-9907
2009
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1
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712623
Nakao
Purification and some properti ...
Vibrio alginolyticus, Vibrio alginolyticus ATCC 17749
J. Gen. Microbiol.
137
1737-1742
1991
2
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