BRENDA - Enzyme Database show
show all sequences of 1.5.1.43

Purification and some properties of carboxynorspermidine synthase participating in a novel biosynthetic pathway for norspermidine inVibrio alginolyticus

Nakao, H.; Shinoda, S.; Yamamoto, S.; J. Gen. Microbiol. 137, 1737-1742 (1991)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
dithiothreitol
about 14fold increase of activity at 20 mM. Without addition of dithiothreitol to the assay mixture, only 7% of the maximum activity is detected in the purified enzyme
Vibrio alginolyticus
additional information
EDTA (1 mM) shows no significant stimulatory effect on enzyme activity
Vibrio alginolyticus
Inhibitors
Inhibitors
Commentary
Organism
Structure
iodoacetamide
24% inhibition at 5 mM
Vibrio alginolyticus
additional information
EDTA (1 mM) shows no significant inhibitory effect on enzyme activity; NAD+, ATP and SPD are not inhibitory at 5 mM
Vibrio alginolyticus
N-ethylmaleimide
83% inhibition at 5 mM
Vibrio alginolyticus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.51
-
NADPH
at pH 7.5 and 37°C
Vibrio alginolyticus
2.97
-
NADH
at pH 7.5 and 37°C
Vibrio alginolyticus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
Ca2+, Mg2+, Fe3+, Fe2+, Cu+, Cu2+, Mn2+, Zn2+, Na+ and K+ at 1 M have no effect on enzyme activity
Vibrio alginolyticus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45100
-
2 * 45100, SDS-PAGE
Vibrio alginolyticus
93500
-
gel filtration
Vibrio alginolyticus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Vibrio alginolyticus
-
-
-
Vibrio alginolyticus ATCC 17749
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate precipitation, Blue Sepharose CL-6B column chromatography, DEAE-Sepharose column chromatography, and hydroxyapatite column chromatography
Vibrio alginolyticus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0167
-
crude enzyme, at pH 7.5 and 37°C
Vibrio alginolyticus
31
-
after 1856fold purification, at pH 7.5 and 37°C
Vibrio alginolyticus
Storage Stability
Storage Stability
Organism
4°C, in 20 mM Tris/HCl, pH 7.5, containing 1 mM dithiothreitol and 0.02% (v/v) NaN3, 1 week, 15% loss of activity
Vibrio alginolyticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADH + H+
NADH is a much poorer cofactor than NADPH
712623
Vibrio alginolyticus
carboxynorspermidine + H2O + NAD+
-
-
-
?
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
-
712623
Vibrio alginolyticus
carboxynorspermidine + H2O + NADP+
-
-
-
?
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
7% activity with putrescine compared to propane-1,3-diamine
712623
Vibrio alginolyticus
carboxyspermidine + H2O + NADP+
-
-
-
?
additional information
does not react with ethylenediamine, cadaverine and D-aspartate 4-semialdehyde
712623
Vibrio alginolyticus
?
-
-
-
-
additional information
does not react with ethylenediamine, cadaverine and D-aspartate 4-semialdehyde
712623
Vibrio alginolyticus ATCC 17749
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homodimer
2 * 45100, SDS-PAGE
Vibrio alginolyticus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Vibrio alginolyticus
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
50
78% and 59% of the maximum activity remain at 30°C and 45°C, respectively, but no activity is observed above 50°C
Vibrio alginolyticus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
7.5
-
Vibrio alginolyticus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.3
8
85% and 18% of the maximum activity are observed at pH 8.0 and 6.3, respectively
Vibrio alginolyticus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
-
the enzyme is unstable in buffers of pH below 6.5. At pH 6.5, 50% loss of activity is observed within 12 h at 4°C
Vibrio alginolyticus
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
NADH is a much poorer cofactor than NADPH
Vibrio alginolyticus
NADPH
-
Vibrio alginolyticus
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Vibrio alginolyticus
isoelectric focusing
4.3
4.2
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
dithiothreitol
about 14fold increase of activity at 20 mM. Without addition of dithiothreitol to the assay mixture, only 7% of the maximum activity is detected in the purified enzyme
Vibrio alginolyticus
additional information
EDTA (1 mM) shows no significant stimulatory effect on enzyme activity
Vibrio alginolyticus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
NADH is a much poorer cofactor than NADPH
Vibrio alginolyticus
NADPH
-
Vibrio alginolyticus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
iodoacetamide
24% inhibition at 5 mM
Vibrio alginolyticus
additional information
EDTA (1 mM) shows no significant inhibitory effect on enzyme activity; NAD+, ATP and SPD are not inhibitory at 5 mM
Vibrio alginolyticus
N-ethylmaleimide
83% inhibition at 5 mM
Vibrio alginolyticus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.51
-
NADPH
at pH 7.5 and 37°C
Vibrio alginolyticus
2.97
-
NADH
at pH 7.5 and 37°C
Vibrio alginolyticus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
Ca2+, Mg2+, Fe3+, Fe2+, Cu+, Cu2+, Mn2+, Zn2+, Na+ and K+ at 1 M have no effect on enzyme activity
Vibrio alginolyticus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45100
-
2 * 45100, SDS-PAGE
Vibrio alginolyticus
93500
-
gel filtration
Vibrio alginolyticus
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, Blue Sepharose CL-6B column chromatography, DEAE-Sepharose column chromatography, and hydroxyapatite column chromatography
Vibrio alginolyticus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0167
-
crude enzyme, at pH 7.5 and 37°C
Vibrio alginolyticus
31
-
after 1856fold purification, at pH 7.5 and 37°C
Vibrio alginolyticus
Storage Stability (protein specific)
Storage Stability
Organism
4°C, in 20 mM Tris/HCl, pH 7.5, containing 1 mM dithiothreitol and 0.02% (v/v) NaN3, 1 week, 15% loss of activity
Vibrio alginolyticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADH + H+
NADH is a much poorer cofactor than NADPH
712623
Vibrio alginolyticus
carboxynorspermidine + H2O + NAD+
-
-
-
?
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
-
712623
Vibrio alginolyticus
carboxynorspermidine + H2O + NADP+
-
-
-
?
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
7% activity with putrescine compared to propane-1,3-diamine
712623
Vibrio alginolyticus
carboxyspermidine + H2O + NADP+
-
-
-
?
additional information
does not react with ethylenediamine, cadaverine and D-aspartate 4-semialdehyde
712623
Vibrio alginolyticus
?
-
-
-
-
additional information
does not react with ethylenediamine, cadaverine and D-aspartate 4-semialdehyde
712623
Vibrio alginolyticus ATCC 17749
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 45100, SDS-PAGE
Vibrio alginolyticus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Vibrio alginolyticus
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
50
78% and 59% of the maximum activity remain at 30°C and 45°C, respectively, but no activity is observed above 50°C
Vibrio alginolyticus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
7.5
-
Vibrio alginolyticus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.3
8
85% and 18% of the maximum activity are observed at pH 8.0 and 6.3, respectively
Vibrio alginolyticus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
-
the enzyme is unstable in buffers of pH below 6.5. At pH 6.5, 50% loss of activity is observed within 12 h at 4°C
Vibrio alginolyticus
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Vibrio alginolyticus
isoelectric focusing
4.3
4.2
Expression
Organism
Commentary
Expression
Vibrio alginolyticus
when Vibrio alginolyticus is grown in the presence of 5 mM norspermidine, the specific activity of the enzyme is reduced by about 70%
down
Expression (protein specific)
Organism
Commentary
Expression
Vibrio alginolyticus
when Vibrio alginolyticus is grown in the presence of 5 mM norspermidine, the specific activity of the enzyme is reduced by about 70%
down
Other publictions for EC 1.5.1.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741488
Kim
The essential role of spermid ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58 / ATCC 33970
ACS Chem. Biol.
11
491-499
2016
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1
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1
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4
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4
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3
3
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742792
Wang
Spermidine inversely influenc ...
Agrobacterium tumefaciens
J. Bacteriol.
198
2682-2691
2016
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1
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1
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2
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5
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2
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1
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1
1
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1
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2
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2
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3
3
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719993
Hanfrey
Alternative spermidine biosynt ...
Campylobacter jejuni
J. Biol. Chem.
286
43301-43312
2011
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7
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1
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1
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704662
Shaw
Evolution and multifarious hor ...
Rhizobium leguminosarum
J. Biol. Chem.
285
14711-14723
2010
-
-
-
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9
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2
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2
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704647
Lee
An alternative polyamine biosy ...
Vibrio cholerae
J. Biol. Chem.
284
9899-9907
2009
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1
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1
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4
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1
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4
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1
1
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-
712623
Nakao
Purification and some properti ...
Vibrio alginolyticus, Vibrio alginolyticus ATCC 17749
J. Gen. Microbiol.
137
1737-1742
1991
2
-
-
-
-
-
3
2
-
1
2
-
-
5
-
-
1
-
-
-
2
1
5
1
1
1
-
-
1
1
1
2
-
1
-
2
-
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2
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3
-
2
-
1
2
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1
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2
1
5
1
1
1
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1
1
1
1
1
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1
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