Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-Chloromercuriphenyl sulfonate | - |
Methanococcus vannielii | |
iodoacetamide | - |
Methanococcus vannielii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
1,5-dihydro-8-hydroxy-5-deazaflavin | pH 7.0, 20°C | Methanococcus vannielii | |
0.008 | - |
8-hydroxy-5-deazaflavin | pH 7.0, 20°C | Methanococcus vannielii | |
0.0104 | - |
NADPH | pH 7.0, 20°C | Methanococcus vannielii | |
0.0137 | - |
NADP+ | pH 7.0, 20°C | Methanococcus vannielii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
2 * 43000, SDS-PAGE | Methanococcus vannielii |
85000 | - |
gel filtration | Methanococcus vannielii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanococcus vannielii | - |
- |
- |
Methanococcus vannielii DSM 1224 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanococcus vannielii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.79 | - |
pH 7.0, 20°C | Methanococcus vannielii |
Storage Stability | Organism |
---|---|
-75°C, 2 months, at low concentration (0.066 mg/ml), in the presence of ethylene glycol, 20% (v/v), no loss of activity | Methanococcus vannielii |
4°C, at low concentration (0.066 mg/ml), in the presence of ethylene glycol, 20% (v/v), 22% of the activity is lost | Methanococcus vannielii |
4°C, at low concentration (0.066 mg/ml), the purified enzyme loses 84% of its activity in 2 days | Methanococcus vannielii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-dihydro-8-hydroxy-5-deazaflavin + NADP+ | the kcat value of the forward reaction is 24 times greater than that of the reverse reaction, thus the production of NADPH at pH 7.0 is more favorable than its consumption | Methanococcus vannielii | 8-hydroxy-5-deazaflavin + NADPH + H+ | - |
r | |
1,5-dihydro-8-hydroxy-5-deazaflavin + NADP+ | the kcat value of the forward reaction is 24 times greater than that of the reverse reaction, thus the production of NADPH at pH 7.0 is more favorable than its consumption | Methanococcus vannielii DSM 1224 | 8-hydroxy-5-deazaflavin + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43000, SDS-PAGE | Methanococcus vannielii |
Synonyms | Comment | Organism |
---|---|---|
8-hydroxy-5-deazaflavin-dependent NADP+ reductase | the enzyme is a component of the formate NADP+ oxidoreductase system of Methanococcus vannielii | Methanococcus vannielii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
17 | 20 | - |
Methanococcus vannielii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.37 | - |
NADP+ | pH 7.0, 20°C | Methanococcus vannielii | |
7.37 | - |
1,5-dihydro-8-hydroxy-5-deazaflavin | pH 7.0, 20°C | Methanococcus vannielii | |
175 | - |
NADPH | pH 7.0, 20°C | Methanococcus vannielii | |
175 | - |
8-hydroxy-5-deazaflavin | pH 7.0, 20°C | Methanococcus vannielii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
reaction with 8-hydroxy-5-deazaflavin + NADPH | Methanococcus vannielii |
7.9 | - |
reaction with 1,5-dihydro-8-hydroxyy-5-deazaflavin + NADP+ | Methanococcus vannielii |