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Literature summary for 1.5.1.38 extracted from
- Vibrio harveyi NADPH-flavin oxidoreductase: cloning, sequencing and overexpression of the gene and purification and characterization of the cloned enzyme (1994), J. Bacteriol., 176, 3552-3558.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Vibrio harveyi |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26312 | - |
1 * 26312, calculated from sequence | Vibrio harveyi |
| 28000 | - |
1 * 28000, SDS-PAGE | Vibrio harveyi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| FMN + NADPH + H+ | Vibrio harveyi | - |
FMNH2 + NADP+ | - |
? |  |
| FMN + NADPH + H+ | Vibrio harveyi MAV / ATCC 33843 | - |
FMNH2 + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio harveyi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vibrio harveyi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 88.4 | - |
23°C, pH not specified in the publication | Vibrio harveyi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| FMN + NADPH + H+ | - |
Vibrio harveyi | FMNH2 + NADP+ | - |
? | |
| FMN + NADPH + H+ | - |
Vibrio harveyi MAV / ATCC 33843 | FMNH2 + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 28000, SDS-PAGE | Vibrio harveyi |
| monomer | 1 * 26312, calculated from sequence | Vibrio harveyi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| flavin reductase P | - |
Vibrio harveyi |
| FRP | - |
Vibrio harveyi |
| NADPH-flavin oxidoreductase | - |
Vibrio harveyi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
assay at | Vibrio harveyi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | contains a tightly bound FMN cofactor | Vibrio harveyi | |
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