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Literature summary for 1.5.1.34 extracted from

  • Xu, Y.; Li, Y.; Li, L.; Zhang, L.; Ding, Z.; Shi, G.
    Reductase-catalyzed tetrahydrobiopterin regeneration alleviates the anti-competitive inhibition of tyrosine hydroxylation by 7,8-dihydrobiopterin (2021), Catal. Sci. Technol., 11, 3128-3140 .
No PubMed abstract available

Application

Application Comment Organism
additional information the purified recombinant enzyme from Escherichia coli is used for 5,6,7,8-tetrahydropteridine (BH4) regeneration to alleviate 7,8-dihydropteridine (BH2) inhibition of L-tyrosine hydroxylation by crude tyrosine hydroxylase (SrTH) from Streptosporangium roseum DSM 43021 as part of a combined whole-cell catalyst, and a series of tyrosine hydroxylase/sepiapterin reductase (TH/SPR) synthesis systems, overview Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
gene nfsB, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) as soluble protein Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
quinoid 7,8-(6H)-dihydropterin + NADH + H+ Escherichia coli non-enzymatic reaction of quinoid 7,8-(6H)-dihydropterine to 7,8-dihydropterin 5,6,7,8-tetrahydropterin + NAD+
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?

Organism

Organism UniProt Comment Textmining
Escherichia coli P38489
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
quinoid 7,8-(6H)-dihydropterin + NADH + H+ non-enzymatic reaction of quinoid 7,8-(6H)-dihydropterine to 7,8-dihydropterin Escherichia coli 5,6,7,8-tetrahydropterin + NAD+
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?

Synonyms

Synonyms Comment Organism
7,8-dihydrobiopterin reductase
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Escherichia coli
DHPR
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Escherichia coli
EcDHPR
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Escherichia coli
NfsB
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Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADH
-
Escherichia coli