Application | Comment | Organism |
---|---|---|
additional information | the purified recombinant enzyme from Escherichia coli is used for 5,6,7,8-tetrahydropteridine (BH4) regeneration to alleviate 7,8-dihydropteridine (BH2) inhibition of L-tyrosine hydroxylation by crude tyrosine hydroxylase (SrTH) from Streptosporangium roseum DSM 43021 as part of a combined whole-cell catalyst, and a series of tyrosine hydroxylase/sepiapterin reductase (TH/SPR) synthesis systems, overview | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
gene nfsB, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) as soluble protein | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
quinoid 7,8-(6H)-dihydropterin + NADH + H+ | Escherichia coli | non-enzymatic reaction of quinoid 7,8-(6H)-dihydropterine to 7,8-dihydropterin | 5,6,7,8-tetrahydropterin + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P38489 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
quinoid 7,8-(6H)-dihydropterin + NADH + H+ | non-enzymatic reaction of quinoid 7,8-(6H)-dihydropterine to 7,8-dihydropterin | Escherichia coli | 5,6,7,8-tetrahydropterin + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
7,8-dihydrobiopterin reductase | - |
Escherichia coli |
DHPR | - |
Escherichia coli |
EcDHPR | - |
Escherichia coli |
NfsB | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Escherichia coli |