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Literature summary for 1.5.1.34 extracted from
- Molecular and enzymatic characterization of two enzymes BmPCD and BmDHPR involving in the regeneration pathway of tetrahydrobiopterin from the silkworm Bombyx mori (2015), Comp. Biochem. Physiol. B, 186, 20-27 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Dhpr, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Bombyx mori |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of lethal mutants of a65 and a60 strains. For a65 (leml/leml) and a60 (al/al), the reaction rate of total proteins is 0.373 and 3.352 microM/min/mg protein, respectively. The expression of BmDhpr is especially activated in the brain and sexual glands of lem mutant and the homozygous lethal mutant of al mutant | Bombyx mori |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0314 | - |
NADH | pH 7.4, 22°C, recombinant enzyme | Bombyx mori |  |
| 0.0729 | - |
NADPH | pH 7.4, 22°C, recombinant enzyme | Bombyx mori | |
| 0.821 | - |
6,7-dimethyl-7,8-dihydropteridine | pH 7.4, 22°C, recombinant enzyme | Bombyx mori | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| a 6,7-dihydropteridine + NAD(P)H + H+ | Bombyx mori | - |
a 5,6,7,8-tetrahydropteridine + NAD(P)+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bombyx mori | - |
several strains | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from fifth larval instar of p50 and ah09 strains, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration | Bombyx mori |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bombyx mori | - |
| fat body | larvaeal | Bombyx mori | - |
| larva | - |
Bombyx mori | - |
| additional information | the expression of BmDhpr is especially activated in the brain and sexual glands of lem mutant and the homozygous lethal mutant of al mutant | Bombyx mori | - |
| sexual gland | - |
Bombyx mori | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6,7-dimethyl-7,8-dihydropteridine + NADH + H+ | NADH is preferred in vitro compared to NADPH | Bombyx mori | 6,7-dimethyl-5,6,7,8-tetrahydropterin + NAD+ | - |
? | |
| a 6,7-dihydropteridine + NAD(P)H + H+ | - |
Bombyx mori | a 5,6,7,8-tetrahydropteridine + NAD(P)+ | - |
? | |
| a 6,7-dihydropteridine + NADPH + H+ | - |
Bombyx mori | a 5,6,7,8-tetrahydropteridine + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 17900, about, sequence calculation | Bombyx mori |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BmDhpr | - |
Bombyx mori |
| DHPR | - |
Bombyx mori |
| dihydropteridine reductase | - |
Bombyx mori |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Bombyx mori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Bombyx mori |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the recombinant BmDHPR exhibits high enzymatic activity and more suitable parameters to the coenzyme of NADH in vitro | Bombyx mori | |
| NAD(P)H | - |
Bombyx mori | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bombyx mori | sequence calculation | - |
9.9 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | tetrahydrobiopterin deficiency is associated with numerous metabolic syndromes and neuropsychological disorders | Bombyx mori |
| metabolism | the enzyme is involved in the De novo pathway and regeneration pathway of tetrahydrobiopterin biosynthesis | Bombyx mori |
| physiological function | the enzyme is important in the regeneration pathway of tetrahydrobiopterin, BH4. Tetrahydrobiopterin (BH4) is an essential cofactor of aromatic amino acid hydroxylases and nitric oxide synthase so that BH4 plays a key role in many biological processes | Bombyx mori |
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