Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Leishmania tarentolae |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme in binary complex with NADPH and in ternary complex with NADPH and methotrexate, 0.02 ml of protein solution containing 20 mg/ml protein, 20 mM Tris-HCl, pH 7.0, mixed with 0.01 ml of a solution containing 4 mM NADPH, 4 mM methotrexate on ice for 1 h, then mixed with 0.01 ml of reservoir solution containing 28% 1,4-butanediol, 12.5 mM cetyl trimethyl ammonium chloride and 100 mM HEPES, pH 7.0, 5 days, 14°C, X-ray diffraction structure determination and analysis of flash frozen crystals at 2.8 A resolution, molecular replacement technique | Leishmania tarentolae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methotrexate | binding structure | Leishmania tarentolae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Leishmania tarentolae | enzyme is used by the organism to bypass antifolate inhibition, catalytic pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania tarentolae | P42556 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Leishmania tarentolae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Biopterin + NADPH | 2-step reaction via intermediate 7,8-dihydrobiopterin | Leishmania tarentolae | 5,6,7,8-Tetrahydrobiopterin + NADP+ | - |
? | |
additional information | enzyme is used by the organism to bypass antifolate inhibition, catalytic pathway | Leishmania tarentolae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | structure analysis | Leishmania tarentolae |
Synonyms | Comment | Organism |
---|---|---|
pteridine reductase | - |
Leishmania tarentolae |
PTR1 | - |
Leishmania tarentolae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | binding structure | Leishmania tarentolae |