BRENDA - Enzyme Database
show all sequences of 1.5.1.30

Trichomonas vaginalis flavin reductase 1 and its role in metronidazole resistance

Leitsch, D.; Janssen, B.; Kolarich, D.; Johnson, P.; Duchene, M.; Mol. Microbiol. 91, 198-208 (2014)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene TVAG_517010, DNA and amino acid sequence determination and analysis, quantitative RT-PCR expression analysis of FR1 in metronidazole-susceptible isolates C1, G3, JH31A and Tv2 , re-introduction of FR1 into an aerobically-resistant strain, transfection of the highly metronidazole-resistant isolate B7268 with the FR1 gene restores metronidazole susceptibility
Trichomonas vaginalis
Inhibitors
Inhibitors
Commentary
Organism
Structure
riboflavin
FR1 inhibition by possibly either by the semiquinone or the fully reduced form
Trichomonas vaginalis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
FMNH2 + NADP+
Trichomonas vaginalis
-
FMN + NADPH + H+
-
-
r
FMNH2 + NADP+
Trichomonas vaginalis G3
-
FMN + NADPH + H+
-
-
r
additional information
Trichomonas vaginalis
FR is a relevant source of hydrogen peroxide but superoxide radical anions are a minor by-product of the reaction
?
-
-
?
additional information
Trichomonas vaginalis G3
FR is a relevant source of hydrogen peroxide but superoxide radical anions are a minor by-product of the reaction
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Trichomonas vaginalis
A2GH85
-
-
Trichomonas vaginalis G3
A2GH85
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
analysis of enzyme FR1 expression in metronidazole-susceptible isolates C1, G3, JH31A and Tv2: the highly metronidazole-resistant cell line of strain C1, C1res, and the clinical isolate B7268 lack detectable FRs. The residual FR activity found in CDC085 and LA1 likely results from alternative FRs that are highly expressed
Trichomonas vaginalis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
FMNH2 + NADP+
-
743307
Trichomonas vaginalis
FMN + NADPH + H+
-
-
-
r
FMNH2 + NADP+
-
743307
Trichomonas vaginalis G3
FMN + NADPH + H+
-
-
-
r
additional information
FR is a relevant source of hydrogen peroxide but superoxide radical anions are a minor by-product of the reaction
743307
Trichomonas vaginalis
?
-
-
-
?
additional information
FR is a relevant source of hydrogen peroxide but superoxide radical anions are a minor by-product of the reaction
743307
Trichomonas vaginalis G3
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
flavin reductase 1
-
Trichomonas vaginalis
FR1
-
Trichomonas vaginalis
TVAG_517010
-
Trichomonas vaginalis
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Trichomonas vaginalis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
assay at
Trichomonas vaginalis
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
FR1 displays only 3% activity when NADH is added instead of NADPH
Trichomonas vaginalis
NADP+
-
Trichomonas vaginalis
NADPH
-
Trichomonas vaginalis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene TVAG_517010, DNA and amino acid sequence determination and analysis, quantitative RT-PCR expression analysis of FR1 in metronidazole-susceptible isolates C1, G3, JH31A and Tv2 , re-introduction of FR1 into an aerobically-resistant strain, transfection of the highly metronidazole-resistant isolate B7268 with the FR1 gene restores metronidazole susceptibility
Trichomonas vaginalis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
FR1 displays only 3% activity when NADH is added instead of NADPH
Trichomonas vaginalis
NADP+
-
Trichomonas vaginalis
NADPH
-
Trichomonas vaginalis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
riboflavin
FR1 inhibition by possibly either by the semiquinone or the fully reduced form
Trichomonas vaginalis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
FMNH2 + NADP+
Trichomonas vaginalis
-
FMN + NADPH + H+
-
-
r
FMNH2 + NADP+
Trichomonas vaginalis G3
-
FMN + NADPH + H+
-
-
r
additional information
Trichomonas vaginalis
FR is a relevant source of hydrogen peroxide but superoxide radical anions are a minor by-product of the reaction
?
-
-
?
additional information
Trichomonas vaginalis G3
FR is a relevant source of hydrogen peroxide but superoxide radical anions are a minor by-product of the reaction
?
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
analysis of enzyme FR1 expression in metronidazole-susceptible isolates C1, G3, JH31A and Tv2: the highly metronidazole-resistant cell line of strain C1, C1res, and the clinical isolate B7268 lack detectable FRs. The residual FR activity found in CDC085 and LA1 likely results from alternative FRs that are highly expressed
Trichomonas vaginalis
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
FMNH2 + NADP+
-
743307
Trichomonas vaginalis
FMN + NADPH + H+
-
-
-
r
FMNH2 + NADP+
-
743307
Trichomonas vaginalis G3
FMN + NADPH + H+
-
-
-
r
additional information
FR is a relevant source of hydrogen peroxide but superoxide radical anions are a minor by-product of the reaction
743307
Trichomonas vaginalis
?
-
-
-
?
additional information
FR is a relevant source of hydrogen peroxide but superoxide radical anions are a minor by-product of the reaction
743307
Trichomonas vaginalis G3
?
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Trichomonas vaginalis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
assay at
Trichomonas vaginalis
General Information
General Information
Commentary
Organism
metabolism
Trichomonas vaginalis has two different enzymatic pathways to remove intracellular oxygen, i.e. NADH oxidase, which is inhibited by metronidazole, and flavin reductase
Trichomonas vaginalis
physiological function
regarding all flavin reductase enzymes in Trichomonas vaginalis, FR1 is responsible for the flavin reductase activity. Hydrogen peroxide is the main if not the single product of FR1. FR1 displays a 10 to 20fold higher affinity to FMN than enzymes FR5 and FR6. Also the affinity of FR1 to FAD is at least 10fold higher than observed with FR5 and FR6 but the Vmax is considerably lower (roughly 33% of the Vmax for FMN). In contrast, FR5 and FR6 display higher affinity for riboflavin than FR1, which is inhibited by riboflavin
Trichomonas vaginalis
General Information (protein specific)
General Information
Commentary
Organism
metabolism
Trichomonas vaginalis has two different enzymatic pathways to remove intracellular oxygen, i.e. NADH oxidase, which is inhibited by metronidazole, and flavin reductase
Trichomonas vaginalis
physiological function
regarding all flavin reductase enzymes in Trichomonas vaginalis, FR1 is responsible for the flavin reductase activity. Hydrogen peroxide is the main if not the single product of FR1. FR1 displays a 10 to 20fold higher affinity to FMN than enzymes FR5 and FR6. Also the affinity of FR1 to FAD is at least 10fold higher than observed with FR5 and FR6 but the Vmax is considerably lower (roughly 33% of the Vmax for FMN). In contrast, FR5 and FR6 display higher affinity for riboflavin than FR1, which is inhibited by riboflavin
Trichomonas vaginalis
Other publictions for EC 1.5.1.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
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2
2
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91
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1
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2
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724343
Chung
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6
6
724769
Haynes
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Escherichia coli
ChemMedChem
6
279-291
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725248
Kozmin
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Escherichia coli
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192
2026-2033
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2
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698992
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Escherichia coli
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284
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1
1
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2
2
699057
Takahashi
Characterization of a flavin r ...
Bacillus subtilis, Bacillus subtilis WU-S2B
J. Biosci. Bioeng.
107
38-41
2009
2
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1
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7
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2
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11
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26
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4
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1
1
1
1
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1
1
1
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1
1
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684711
Jawanda
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Vibrio harveyi
Arch. Biochem. Biophys.
472
51-57
2008
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1
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1
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685230
Jawanda
Vibrio harveyi flavin reductas ...
Vibrio harveyi
Biochemistry
47
368-377
2008
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1
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1
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689388
Tu
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Aliivibrio fischeri, Vibrio harveyi
Photochem. Photobiol. Sci.
7
183-188
2008
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684610
Liu
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Luteovulum sphaeroides, Luteovulum sphaeroides AS1.1737
Appl. Microbiol. Biotechnol.
76
1271-1279
2007
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689909
Vorontsov
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Shigella flexneri
Protein Sci.
16
2483-2490
2007
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671610
Li
Activity coupling of Vibrio ha ...
Vibrio harveyi
Arch. Biochem. Biophys.
454
26-31
2006
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672105
Li
Thermodynamic analysis of the ...
Vibrio harveyi
Biochemistry
45
14781-14787
2006
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672184
Deller
Characterization of a thermost ...
Bacillus subtilis
Biochemistry
45
7083-7091
2006
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678187
Okai
Crystal structures of the shor ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7
Biochemistry
45
5103-5110
2006
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16
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657786
Gao
Altered mechanism of the alkan ...
Escherichia coli
Biochem. Biophys. Res. Commun.
331
1137-1145
2005
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658108
Lei
Redox potential and equilibria ...
Aliivibrio fischeri, Vibrio harveyi
Biochemistry
44
261-267
2005
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2
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1
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2
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7
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2
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5
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