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Literature summary for 1.5.1.30 extracted from

  • Jawanda, N.; Ahmed, K.; Tu, S.C.
    Vibrio harveyi flavin reductase-luciferase fusion protein mimics a single-component bifunctional monooxygenase (2008), Biochemistry, 47, 368-377.
    View publication on PubMed
Search for more literature for 1.5.1.30

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Vibrio harveyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 FMN native enzyme, at 23°C in 50 mM phosphate buffer, pH 7.0 Vibrio harveyi
0.02
-
 NADPH native enzyme, at 23°C in 50 mM phosphate buffer, pH 7.0 Vibrio harveyi
0.072
-
 FMN enzyme fused to luciferase, at 23°C in 50 mM phosphate buffer, pH 7.0 Vibrio harveyi
0.31
-
 NADPH enzyme fused to luciferase, at 23°C in 50 mM phosphate buffer, pH 7.0 Vibrio harveyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26300
-
 2 * 26300, gel filtration Vibrio harveyi

Organism

Organism UniProt Comment Textmining
Vibrio harveyi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
the flavin reductase-luciferase fusion protein is purified using DEAE-cellulose DE-52 column chromatography, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration Vibrio harveyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FMN + NADPH
-
 Vibrio harveyi FMNH2 + NADP+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 26300, gel filtration Vibrio harveyi

Synonyms

Synonyms Comment Organism
FRP
-
 Vibrio harveyi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.7
-
 FMN enzyme fused to luciferase, at 23°C in 50 mM phosphate buffer, pH 7.0 Vibrio harveyi
37.5
-
 FMN native enzyme, at 23°C in 50 mM phosphate buffer, pH 7.0 Vibrio harveyi

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Vibrio harveyi
NADPH
-
 Vibrio harveyi