Cloned (Comment) | Organism |
---|---|
expression of wild-type enzyme and mutant R203A in Escherichia coli | Vibrio harveyi |
expression of wild-type enzyme in Escherichia coli | Aliivibrio fischeri |
Protein Variants | Comment | Organism |
---|---|---|
R203A | site-directed mutagenesis, no reversibility of the reduction of FMN, the half-reaction | Vibrio harveyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | substrate and cofactor binding, reaction kinetics | Aliivibrio fischeri | |
additional information | - |
additional information | substrate and cofactor binding, reaction kinetics | Vibrio harveyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aliivibrio fischeri | - |
- |
- |
Vibrio harveyi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli to homogeneity | Aliivibrio fischeri |
recombinant wild-type and mutant enzyme from Escherichia coli to homogeneity | Vibrio harveyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
riboflavin + NAD(P)H | redox potential of the irreversible reductive half-reaction | Aliivibrio fischeri | reduced riboflavin + NADP+ | - |
? | |
riboflavin + NADPH | redox potential and equilibria in the reversible reductive half-reaction | Vibrio harveyi | reduced riboflavin + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Aliivibrio fischeri |
dimer | - |
Vibrio harveyi |
Synonyms | Comment | Organism |
---|---|---|
FRP | - |
Aliivibrio fischeri |
FRP | - |
Vibrio harveyi |
More | see also EC 1.5.1.29 | Aliivibrio fischeri |
More | see also EC 1.5.1.29 | Vibrio harveyi |
NADPH-FMN oxidoreductase | - |
Vibrio harveyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Aliivibrio fischeri |
7 | - |
assay at | Vibrio harveyi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | 1 FMN bound per subunit of the dimeric enzyme | Aliivibrio fischeri | |
FMN | 1 FMN bound per subunit of the dimeric enzyme | Vibrio harveyi | |
NAD(P)H | - |
Aliivibrio fischeri | |
NADPH | - |
Vibrio harveyi |