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Literature summary for 1.5.1.30 extracted from

  • Lei, B.; Wang, H.; Yu, Y.; Tu, S.C.
    Redox potential and equilibria in the reductive half-reaction of Vibrio harveyi NADPH-FMN oxidoreductase (2005), Biochemistry, 44, 261-267.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type enzyme and mutant R203A in Escherichia coli Vibrio harveyi
expression of wild-type enzyme in Escherichia coli Aliivibrio fischeri

Protein Variants

Protein Variants Comment Organism
R203A site-directed mutagenesis, no reversibility of the reduction of FMN, the half-reaction Vibrio harveyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information substrate and cofactor binding, reaction kinetics Aliivibrio fischeri
additional information
-
additional information substrate and cofactor binding, reaction kinetics Vibrio harveyi

Organism

Organism UniProt Comment Textmining
Aliivibrio fischeri
-
-
-
Vibrio harveyi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli to homogeneity Aliivibrio fischeri
recombinant wild-type and mutant enzyme from Escherichia coli to homogeneity Vibrio harveyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
riboflavin + NAD(P)H redox potential of the irreversible reductive half-reaction Aliivibrio fischeri reduced riboflavin + NADP+
-
?
riboflavin + NADPH redox potential and equilibria in the reversible reductive half-reaction Vibrio harveyi reduced riboflavin + NADP+
-
?

Subunits

Subunits Comment Organism
dimer
-
Aliivibrio fischeri
dimer
-
Vibrio harveyi

Synonyms

Synonyms Comment Organism
FRP
-
Aliivibrio fischeri
FRP
-
Vibrio harveyi
More see also EC 1.5.1.29 Aliivibrio fischeri
More see also EC 1.5.1.29 Vibrio harveyi
NADPH-FMN oxidoreductase
-
Vibrio harveyi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Aliivibrio fischeri
7
-
assay at Vibrio harveyi

Cofactor

Cofactor Comment Organism Structure
FMN 1 FMN bound per subunit of the dimeric enzyme Aliivibrio fischeri
FMN 1 FMN bound per subunit of the dimeric enzyme Vibrio harveyi
NAD(P)H
-
Aliivibrio fischeri
NADPH
-
Vibrio harveyi