Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli | |
NADPH | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000016 | - |
FMN | 25°C, single-enzyme assay | Escherichia coli | |
0.00013 | - |
FMN | 25°C, enzyme in complex with monooxygenase SsuD, presence of octanesulfonate | Escherichia coli | |
0.0054 | - |
NADPH | 25°C, single-enzyme assay | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
enzyme SsuE is part of alkanesulfonate monooxygenase system, together with a monooxygenase enzyme SsuD | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
reduced riboflavin + NADP+ = riboflavin + NADPH + H+ | single-enzyme kinetic assay, SsuE follows ordered sequential mechanism with NADPH as the first substrate to bind and NADP+ as the last product to leave. In presence of monooxygenase SsuD and octanesulfonate mechanism is altered to rapid equilibrium ordered mechanism, and Km-value for FMN increases 10fold | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FMN + NADPH | - |
Escherichia coli | FMNH2 + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alkanesulfonate FMN reductase | - |
Escherichia coli |
SsuE | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
FMN | 25°C, enzyme in complex with monooxygenase SsuD, presence of octanesulfonate | Escherichia coli | |
1.9 | - |
FMN | 25°C, single-enzyme assay | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Escherichia coli | |
NADPH | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0035 | - |
NADPH | 25°C, enzyme in complex with monooxygenase SsuD, presence of octanesulfonate | Escherichia coli | |
0.0039 | - |
NADPH | 25°C, single-enzyme assay | Escherichia coli | |
0.0139 | - |
NADP+ | 25°C, single-enzyme assay | Escherichia coli |