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Literature summary for 1.5.1.30 extracted from
- Purification, characterization, and overexpression of flavin reductase involved in dibenzothiophene desulfurization by Rhodococcus erythropolis D-1 (2001), Appl. Environ. Microbiol., 67, 1179-1184.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Rhodococcus erythropolis |
General Stability
| General Stability | Organism |
|---|---|
| stable at temperatures below 50°C | Rhodococcus erythropolis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 7-hydroxycoumarin | - |
Rhodococcus erythropolis |  |
| Ag+ | - |
Rhodococcus erythropolis | |
| Cu2+ | - |
Rhodococcus erythropolis | |
| Hg+ | - |
Rhodococcus erythropolis | |
| NEM | when the enzyme is preincubated with NEM and NADH in the absence of FMN | Rhodococcus erythropolis | |
| p-chloromercuribenzoic acid | - |
Rhodococcus erythropolis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0108 | - |
FMN | - |
Rhodococcus erythropolis | |
| 0.208 | - |
NADH | - |
Rhodococcus erythropolis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
4 * 22000, SDS-PAGE | Rhodococcus erythropolis |
| 86000 | - |
gel filtration | Rhodococcus erythropolis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus erythropolis | - |
D1 | - |
| Rhodococcus erythropolis D1 | - |
D1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhodococcus erythropolis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 122 | - |
- |
Rhodococcus erythropolis |
| 133 | - |
flavin reductase from recombinant Escherichia coli | Rhodococcus erythropolis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| FMN + NADPH | - |
Rhodococcus erythropolis | FMNH2 + NADP+ | - |
? | |
| FMN + NADPH | - |
Rhodococcus erythropolis D1 | FMNH2 + NADP+ | - |
? | |
| riboflavin-5-phosphate + NADH + H+ | - |
Rhodococcus erythropolis | reduced FMN + NAD+ | - |
r | |
| riboflavin-5-phosphate + NADH + H+ | - |
Rhodococcus erythropolis D1 | reduced FMN + NAD+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 22000, SDS-PAGE | Rhodococcus erythropolis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
enzyme assay | Rhodococcus erythropolis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
enzyme assay | Rhodococcus erythropolis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | - |
Rhodococcus erythropolis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | without any chromophore like flavin, NADPH is inert | Rhodococcus erythropolis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00338 | - |
7-hydroxycoumarin | - |
Rhodococcus erythropolis | |
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