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Literature summary for 1.5.1.30 extracted from
- Vibrio harveyi NADPH-flavin oxidoreductase: cloning, sequencing and overexpression of the gene and purification and characterization of the cloned enzyme (1994), J. Bacteriol., 176, 3552-3558.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Vibrio harveyi |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
FMN | - |
Vibrio harveyi |  |
| 0.02 | - |
NADPH | - |
Vibrio harveyi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26320 | - |
calculated from sequence of cloned DNA | Vibrio harveyi |
| 28000 | - |
1 * 28000, wild type and cloned, SDS-PAGE | Vibrio harveyi |
| 33000 | - |
gel filtration | Vibrio harveyi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio harveyi | - |
- |
- |
| Vibrio harveyi MAV / ATCC 33843 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| FMN-agarose chromatography as a key step | Vibrio harveyi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 88 | - |
- |
Vibrio harveyi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| FMN + NADPH | - |
Vibrio harveyi | FMNH2 + NADP+ | - |
? | |
| FMN + NADPH | - |
Vibrio harveyi MAV / ATCC 33843 | FMNH2 + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 28000, wild type and cloned, SDS-PAGE | Vibrio harveyi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADPH:flavin oxidoreductase | - |
Vibrio harveyi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
enzyme assay | Vibrio harveyi |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 36 | - |
FMN | - |
Vibrio harveyi | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | tightly bound | Vibrio harveyi | |
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