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Literature summary for 1.5.1.3 extracted from

  • Sah, S.; Shah, R.; Govindan, A.; Varada, R.; Rex, K.; Varshney, U.
    Utilisation of 10-formyldihydrofolate as substrate by dihydrofolate reductase (DHFR) and 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) tranformylase/IMP cyclohydrolase (PurH) in Escherichia coli (2018), Microbiology, 164, 982-991 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
10-formyl-dihydrofolate pH 7.5, temperature not specified in the publication Escherichia coli
0.0055
-
7,8-dihydrofolate pH 7.5, temperature not specified in the publication Escherichia coli
0.0074
-
NADPH pH 7.5, temperature not specified in the publication, cosubstrate: 10-formyl-dihydrofolate Escherichia coli
0.008
-
NADPH pH 7.5, temperature not specified in the publication, cosubstrate: 7,8-dihydrofolate Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyl-dihydrofolate + NADPH + H+ Escherichia coli the enzyme is involved in the folate recycling pathway 10-formyl-tetrahydrofolate + NADP+
-
?
10-formyl-dihydrofolate + NADPH + H+ Escherichia coli K12 the enzyme is involved in the folate recycling pathway 10-formyl-tetrahydrofolate + NADP+
-
?
7,8-dihydrofolate + NADPH + H+ Escherichia coli the enzyme is involved in the folate recycling pathway 5,6,7,8-tetrahydrofolate + NADP+
-
?
7,8-dihydrofolate + NADPH + H+ Escherichia coli K12 the enzyme is involved in the folate recycling pathway 5,6,7,8-tetrahydrofolate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0ABQ4
-
-
Escherichia coli K12 P0ABQ4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyl-dihydrofolate + NADPH + H+
-
Escherichia coli 10-formyl-tetrahydrofolate + NADP+
-
?
10-formyl-dihydrofolate + NADPH + H+ the enzyme is involved in the folate recycling pathway Escherichia coli 10-formyl-tetrahydrofolate + NADP+
-
?
10-formyl-dihydrofolate + NADPH + H+
-
Escherichia coli K12 10-formyl-tetrahydrofolate + NADP+
-
?
10-formyl-dihydrofolate + NADPH + H+ the enzyme is involved in the folate recycling pathway Escherichia coli K12 10-formyl-tetrahydrofolate + NADP+
-
?
7,8-dihydrofolate + NADPH + H+
-
Escherichia coli 5,6,7,8-tetrahydrofolate + NADP+
-
?
7,8-dihydrofolate + NADPH + H+ the enzyme is involved in the folate recycling pathway Escherichia coli 5,6,7,8-tetrahydrofolate + NADP+
-
?
7,8-dihydrofolate + NADPH + H+
-
Escherichia coli K12 5,6,7,8-tetrahydrofolate + NADP+
-
?
7,8-dihydrofolate + NADPH + H+ the enzyme is involved in the folate recycling pathway Escherichia coli K12 5,6,7,8-tetrahydrofolate + NADP+
-
?

Subunits

Subunits Comment Organism
? x * 22000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
DHFR
-
Escherichia coli
folA
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.05
-
NADPH pH 7.5, temperature not specified in the publication, cosubstrate: 10-formyl-dihydrofolate Escherichia coli
4.6
-
10-formyl-dihydrofolate pH 7.5, temperature not specified in the publication Escherichia coli
22.8
-
7,8-dihydrofolate pH 7.5, temperature not specified in the publication Escherichia coli
28.1
-
NADPH pH 7.5, temperature not specified in the publication, cosubstrate: 7,8-dihydrofolate Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli

General Information

General Information Comment Organism
malfunction growth deficiency phenotypes (in un-supplemented M9 minimal medium containing thymidine) are direct consequences of the gene deletions Escherichia coli
metabolism the enzyme is involved in the folate recycling pathway Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
547.3
-
NADPH pH 7.5, temperature not specified in the publication, cosubstrate: 10-formyl-dihydrofolate Escherichia coli
1533.3
-
10-formyl-dihydrofolate pH 7.5, temperature not specified in the publication Escherichia coli
3512.5
-
NADPH pH 7.5, temperature not specified in the publication, cosubstrate: 7,8-dihydrofolate Escherichia coli
4560
-
7,8-dihydrofolate pH 7.5, temperature not specified in the publication Escherichia coli