Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
10-formyl-dihydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli | |
0.0055 | - |
7,8-dihydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli | |
0.0074 | - |
NADPH | pH 7.5, temperature not specified in the publication, cosubstrate: 10-formyl-dihydrofolate | Escherichia coli | |
0.008 | - |
NADPH | pH 7.5, temperature not specified in the publication, cosubstrate: 7,8-dihydrofolate | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyl-dihydrofolate + NADPH + H+ | Escherichia coli | the enzyme is involved in the folate recycling pathway | 10-formyl-tetrahydrofolate + NADP+ | - |
? | |
10-formyl-dihydrofolate + NADPH + H+ | Escherichia coli K12 | the enzyme is involved in the folate recycling pathway | 10-formyl-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH + H+ | Escherichia coli | the enzyme is involved in the folate recycling pathway | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH + H+ | Escherichia coli K12 | the enzyme is involved in the folate recycling pathway | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0ABQ4 | - |
- |
Escherichia coli K12 | P0ABQ4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyl-dihydrofolate + NADPH + H+ | - |
Escherichia coli | 10-formyl-tetrahydrofolate + NADP+ | - |
? | |
10-formyl-dihydrofolate + NADPH + H+ | the enzyme is involved in the folate recycling pathway | Escherichia coli | 10-formyl-tetrahydrofolate + NADP+ | - |
? | |
10-formyl-dihydrofolate + NADPH + H+ | - |
Escherichia coli K12 | 10-formyl-tetrahydrofolate + NADP+ | - |
? | |
10-formyl-dihydrofolate + NADPH + H+ | the enzyme is involved in the folate recycling pathway | Escherichia coli K12 | 10-formyl-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH + H+ | - |
Escherichia coli | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH + H+ | the enzyme is involved in the folate recycling pathway | Escherichia coli | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH + H+ | - |
Escherichia coli K12 | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH + H+ | the enzyme is involved in the folate recycling pathway | Escherichia coli K12 | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 22000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
DHFR | - |
Escherichia coli |
folA | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.05 | - |
NADPH | pH 7.5, temperature not specified in the publication, cosubstrate: 10-formyl-dihydrofolate | Escherichia coli | |
4.6 | - |
10-formyl-dihydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli | |
22.8 | - |
7,8-dihydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli | |
28.1 | - |
NADPH | pH 7.5, temperature not specified in the publication, cosubstrate: 7,8-dihydrofolate | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | growth deficiency phenotypes (in un-supplemented M9 minimal medium containing thymidine) are direct consequences of the gene deletions | Escherichia coli |
metabolism | the enzyme is involved in the folate recycling pathway | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
547.3 | - |
NADPH | pH 7.5, temperature not specified in the publication, cosubstrate: 10-formyl-dihydrofolate | Escherichia coli | |
1533.3 | - |
10-formyl-dihydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli | |
3512.5 | - |
NADPH | pH 7.5, temperature not specified in the publication, cosubstrate: 7,8-dihydrofolate | Escherichia coli | |
4560 | - |
7,8-dihydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli |