Cloned (Comment) | Organism |
---|---|
a dihydrofolate reductase-like sequence, DHFRLS, is encoded on chromosome 4, orfs 3139757-3140178 and 413244-415704, phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis. RT-qPCR analysis shows no evidence of expression of the DHFRLS in rat | Rattus norvegicus |
a dihydrofolate reductase-like sequence, DHFRLS, is encoded on chromosome 9, orf 31567433:31568480, phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis | Mus musculus |
phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | DHFRLS in wild black rat has an intact open reading frame due to a 2 bp deletion compared to the brown rat or the Brown Norway rat | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
cytoplasm | - |
Mus musculus | 5737 | - |
cytoplasm | - |
Rattus norvegicus | 5737 | - |
mitochondrion | - |
Homo sapiens | 5739 | - |
mitochondrion | the source of mitochondrial DHFR activity is parental DHFR in mouse | Mus musculus | 5739 | - |
mitochondrion | the source of mitochondrial DHFR activity is parental DHFR in rat | Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH + H+ | Homo sapiens | - |
5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
7,8-dihydrofolate + NADPH + H+ | Mus musculus | - |
5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
7,8-dihydrofolate + NADPH + H+ | Rattus norvegicus | - |
5,6,7,8-tetrahydrofolate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00374 | - |
- |
Homo sapiens | Q86XF0 | - |
- |
Mus musculus | P00375 | - |
- |
Rattus norvegicus | Q920D2 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme partially by purification of mitochondria | Mus musculus |
native enzyme partially by purification of mitochondria | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
4T1 cell | enzyme activity is highly increased in the cancer cell line | Mus musculus | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Homo sapiens | - |
liver | - |
Mus musculus | - |
liver | - |
Rattus norvegicus | - |
NRK cell | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.005 | - |
liver cell mitochondria, pH 7.5, temperature not specified in the publication | Mus musculus |
0.006 | - |
liver cell mitochondria, pH 7.5, temperature not specified in the publication | Rattus norvegicus |
0.009 | - |
kidney cell mitochondria, pH 7.5, temperature not specified in the publication | Rattus norvegicus |
0.044 | - |
mitochondria of cancer cell line 4T1, pH 7.5, temperature not specified in the publication | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH + H+ | - |
Homo sapiens | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
7,8-dihydrofolate + NADPH + H+ | - |
Mus musculus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
7,8-dihydrofolate + NADPH + H+ | - |
Rattus norvegicus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 21000, SDS-PAGE | Mus musculus |
? | x * 21000, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
DHFR | - |
Mus musculus |
DHFR | - |
Rattus norvegicus |
DHFR | - |
Homo sapiens |
DHFR2 | - |
Homo sapiens |
DHFRL1 | - |
Homo sapiens |
DHFRLS | - |
Mus musculus |
dihydrofolate reductase-like | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
7.5 | - |
assay at | Mus musculus |
7.5 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens | |
NADP+ | - |
Mus musculus | |
NADP+ | - |
Rattus norvegicus | |
NADPH | - |
Homo sapiens | |
NADPH | - |
Mus musculus | |
NADPH | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
evolution | identification of a second functional dihydrofolate reductase enzyme in humans, DHFRL1. RNA-mediated DHFR duplication events occur across the mammal tree. Dihydrofolate reductase activity is also a feature of the mitochondria in both rat and mouse but this is not due to a second enzyme. Humans have evolved the need for two separate enzymes, while laboratory rats and mice have just one. RNA-mediated DHFR duplicates in brown rat and mouse are likely to be processed pseudogenes | Homo sapiens |
evolution | identification of a second functional dihydrofolate reductase enzyme in humans, DHFRL1. RNA-mediated DHFR duplication events occur across the mammal tree. Dihydrofolate reductase activity is also a feature of the mitochondria in both rat and mouse but this is not due to a second enzyme. Humans have evolved the need for two separate enzymes, while laboratory rats and mice have just one. RNA-mediated DHFR duplicates in brown rat and mouse are likely to be processed pseudogenes | Mus musculus |
evolution | identification of a second functional dihydrofolate reductase enzyme in humans, DHFRL1. RNA-mediated DHFR duplication events occur across the mammal tree. Dihydrofolate reductase activity is also a feature of the mitochondria in both rat and mouse but this is not due to a second enzyme. Humans have evolved the need for two separate enzymes, while laboratory rats and mice have just one. RNA-mediated DHFR duplicates in brown rat and mouse are likely to be processed pseudogenes | Rattus norvegicus |
physiological function | dihydrofolate reductase (DHFR) is an enzyme from the folate one-carbon metabolism pathway that plays a role in drug resistance and in reducing the synthetic supplement folic acid and 7,8-dihydrofolate to the active form, tetrahydrofolate | Homo sapiens |
physiological function | dihydrofolate reductase (DHFR) is an enzyme from the folate one-carbon metabolism pathway that plays a role in drug resistance and in reducing the synthetic supplement folic acid and 7,8-dihydrofolate to the active form, tetrahydrofolate | Mus musculus |
physiological function | dihydrofolate reductase (DHFR) is an enzyme from the folate one-carbon metabolism pathway that plays a role in drug resistance and in reducing the synthetic supplement folic acid and 7,8-dihydrofolate to the active form, tetrahydrofolate | Rattus norvegicus |