Literature summary for 1.5.1.3 extracted from

Ohmae, E.; Fukumizu, Y.; Iwakura, M.; Gekko, K.
Effects of mutation at methionine-42 of Escherichia coli dihydrofolate reductase on stability and function: implication of hydrophobic interactions (2005), J. Biochem., 137, 643-652.

Search for more literature for 1.5.1.3

Protein Variants

Protein Variants	Comment	Organism
M42A	45% decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation	Escherichia coli
M42C	slight decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation	Escherichia coli
M42E	6fold decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation	Escherichia coli
M42G	45% decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation	Escherichia coli
M42H	40% decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation	Escherichia coli
M42I	slight increase in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation	Escherichia coli
M42L	slight decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation	Escherichia coli
M42P	50% increase in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation	Escherichia coli
M42Q	45% decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation	Escherichia coli
M42S	45% decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation	Escherichia coli
M42T	slight increase in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation	Escherichia coli
M42V	slight decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation	Escherichia coli
M42W	6fold decrease in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation	Escherichia coli
M42Y	30% increase in ratio kcat/Km, analysis of thermodynamic parameters for urea denaturation	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00061	-	7,8-dihydrofolate	mutant M42I, pH 7.0, 25°C	Escherichia coli
0.00082	-	7,8-dihydrofolate	mutant M42T, pH 7.0, 25°C	Escherichia coli
0.00083	-	7,8-dihydrofolate	mutant M42P, pH 7.0, 25°C	Escherichia coli
0.00091	-	7,8-dihydrofolate	mutant M42V, pH 7.0, 25°C	Escherichia coli
0.00112	-	7,8-dihydrofolate	mutant M42L, pH 7.0, 25°C	Escherichia coli
0.00118	-	7,8-dihydrofolate	mutant M42G, pH 7.0, 25°C	Escherichia coli
0.0013	-	7,8-dihydrofolate	wild-type, pH 7.0, 25°C	Escherichia coli
0.0013	-	7,8-dihydrofolate	mutant M42A, pH 7.0, 25°C	Escherichia coli
0.00178	-	7,8-dihydrofolate	mutant M42Y, pH 7.0, 25°C	Escherichia coli
0.00193	-	7,8-dihydrofolate	mutant M42H, pH 7.0, 25°C	Escherichia coli
0.00194	-	7,8-dihydrofolate	mutant M42C, pH 7.0, 25°C	Escherichia coli
0.00227	-	7,8-dihydrofolate	mutant M42Q, pH 7.0, 25°C	Escherichia coli
0.00227	-	7,8-dihydrofolate	mutant M42S, pH 7.0, 25°C	Escherichia coli
0.00597	-	7,8-dihydrofolate	mutant M42E, pH 7.0, 25°C	Escherichia coli
0.045	-	7,8-dihydrofolate	mutant M42W, pH 7.0, 25°C	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7,8-dihydrofolate + NADPH	-	Escherichia coli	5,6,7,8-tetrahydrofolate + NADP+	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
12.4	-	7,8-dihydrofolate	mutant M42I, pH 7.0, 25°C	Escherichia coli
13	-	7,8-dihydrofolate	mutant M42G, pH 7.0, 25°C	Escherichia coli
14	-	7,8-dihydrofolate	mutant M42A, pH 7.0, 25°C	Escherichia coli
15.7	-	7,8-dihydrofolate	mutant M42V, pH 7.0, 25°C	Escherichia coli
15.9	-	7,8-dihydrofolate	mutant M42E, pH 7.0, 25°C	Escherichia coli
18.7	-	7,8-dihydrofolate	mutant M42T, pH 7.0, 25°C	Escherichia coli
18.9	-	7,8-dihydrofolate	mutant M42L, pH 7.0, 25°C	Escherichia coli
22.6	-	7,8-dihydrofolate	mutant M42S, pH 7.0, 25°C	Escherichia coli
24	-	7,8-dihydrofolate	mutant M42P, pH 7.0, 25°C	Escherichia coli
24.3	-	7,8-dihydrofolate	mutant M42Q, pH 7.0, 25°C	Escherichia coli
24.6	-	7,8-dihydrofolate	wild-type, pH 7.0, 25°C	Escherichia coli
26.6	-	7,8-dihydrofolate	mutant M42H, pH 7.0, 25°C	Escherichia coli
30.4	-	7,8-dihydrofolate	mutant M42C, pH 7.0, 25°C	Escherichia coli
45.5	-	7,8-dihydrofolate	mutant M42Y, pH 7.0, 25°C	Escherichia coli
89	-	7,8-dihydrofolate	mutant M42W, pH 7.0, 25°C	Escherichia coli
104.6	-	7,8-dihydrofolate	mutant M42W, pH 7.0, 25°C	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Escherichia coli

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Release 2023.1 (January 2023)