Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(-)-epicatechin gallate | competitive to 7,8-dihydrofolate, formation of a slow dissociation ternary complex by the reaction of NADPH with the enzyme-inhibitor complex. Ionization state of E30 is critical for inhibitory activity | Bos taurus | |
(-)-epigallocatechin gallate | competitive to 7,8-dihydrofolate, formation of a slow dissociation ternary complex by the reaction of NADPH with the enzyme-inhibitor complex. Ionization state of E30 is critical for inhibitory activity | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00043 | - |
NADPH | 25°C, pH 7.6 | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | - |
Bos taurus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.1 | - |
NADPH | 25°C, pH 7.6 | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Bos taurus | |
NADPH | - |
Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000082 | - |
(-)-epicatechin gallate | 25°C, pH 7.6 | Bos taurus | |
0.00012 | - |
(-)-epigallocatechin gallate | 25°C, pH 7.6 | Bos taurus |