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Literature summary for 1.5.1.3 extracted from

  • Chitnumsub, P.; Yuvaniyama, J.; Vanichtanankul, J.; Kamchonwongpaisan, S.; Walkinshaw, M.D.; Yuthavong, Y.
    Characterization, crystallization and preliminary X-ray analysis of bifunctional dihydrofolate reductase-thymidylate synthase from Plasmodium falciparum (2004), Acta Crystallogr. Sect. D, 60, 780-783.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) pLysS Plasmodium falciparum

Crystallization (Commentary)

Crystallization (Comment) Organism
modified microbatch method with PRG 4000 as the primary precipitating agent, crystals belong to the orthorhombic space group P2(1)2(1)2(1), with two molecules per asymmetric unit and 52% solvent content Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydrofolate + NADPH
-
Plasmodium falciparum 5,6,7,8-tetrahydrofolate + NADP+
-
?

Subunits

Subunits Comment Organism
More a major band at 35000 Da and minor bands at 35000 Da and 42000 Da detected on SDS-PAGE Plasmodium falciparum

Synonyms

Synonyms Comment Organism
dihydrofolate reductase
-
Plasmodium falciparum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Plasmodium falciparum