Activating Compound | Comment | Organism | Structure |
---|---|---|---|
KCl | recombinant enzyme, 1.4fold activation at 40-200 mM | Thermotoga maritima | |
NaCl | recombinant enzyme, 1.4fold activation at 40-200 mM | Thermotoga maritima | |
Urea | recombinant enzyme, 7 M | Thermotoga maritima |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermotoga maritima |
General Stability | Organism |
---|---|
substrates 7,8-dihydrofolate and NADPH stabilize against thermal inactivation | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | recombinant enzyme, above 20 mM | Thermotoga maritima | |
Guanidine-HCl | recombinant enzyme, 0.5 M | Thermotoga maritima | |
methotrexate | recombinant enzyme | Thermotoga maritima | |
Sodium dodecyl sulfate | recombinant enzyme, 0.1 M | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
7,8-dihydrofolate | recombinant enzyme | Thermotoga maritima | |
0.004 | - |
NADPH | recombinant enzyme | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
2 * 19000, recombinant enzyme, SDS-PAGE, mass spectroscopy | Thermotoga maritima |
36000 | - |
about, recombinant enzyme, gel filtration | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
thermophilic bacterium | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Thermotoga maritima |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
173 | - |
recombinant enzyme | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | - |
Thermotoga maritima | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 19000, recombinant enzyme, SDS-PAGE, mass spectroscopy | Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life: about 60 min, half-life with NADPH: more than 360 min, half-life with 7,8-dihydrofolate: more than 240 min, half-life with 1M urea: 26-28 min | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme-dihydrofolate complex is soluble at basic pH and precipitates reversibly at acidic pH | Thermotoga maritima |
6.3 | - |
recombinant enzyme, 60°C | Thermotoga maritima |
6.5 | - |
recombinant enzyme, 37°C | Thermotoga maritima |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Thermotoga maritima |