Activating Compound | Comment | Organism | Structure |
---|---|---|---|
KCl | 2.5-3fold increase in activity at 0.1 M | Trypanosoma brucei | |
NaCl | 2.5-3fold increase in activity at 0.1 M | Trypanosoma brucei | |
Urea | stimulates 1.5fold at 0.75-1.5 M | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methotrexate | - |
Trypanosoma brucei | |
pyrimethamine | - |
Trypanosoma brucei | |
trimethoprim | - |
Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
7,8-dihydrofolate | recombinant bifunctional enzyme | Trypanosoma brucei | |
0.0009 | - |
NADPH | recombinant bifunctional enzyme | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
trimethoprim-resistant strain | - |
Trypanosoma brucei | - |
bifuntional enzyme with dihydrofolate reductase and thymidylate synthase activity | - |
Purification (Comment) | Organism |
---|---|
recombinant bifunctional protein from Escherichia coli | Trypanosoma brucei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12 | - |
recombinant bifunctional enzyme | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | - |
Trypanosoma brucei | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHFR-TS | - |
Trypanosoma brucei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
7,8-dihydrofolate | recombinant bifunctional enzyme | Trypanosoma brucei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | broad maximum, recombinant bifunctional enzyme | Trypanosoma brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Trypanosoma brucei |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | overview | Trypanosoma brucei | |
0.00000023 | - |
methotrexate | recombinant bifunctional enzyme | Trypanosoma brucei | |
0.00000028 | - |
pyrimethamine | recombinant bifunctional enzyme | Trypanosoma brucei |