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Literature summary for 1.5.1.3 extracted from

  • Gamarro, F.; Yu, P.L.; Zhao, J.; Edman, U.; Greene, P.J.; Santi, D.
    Trypanosoma brucei dihydrofolate reductase-thymidylate synthase: gene isolation and expression and characterization of the enzyme (1995), Mol. Biochem. Parasitol., 72, 11-22.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
KCl 2.5-3fold increase in activity at 0.1 M Trypanosoma brucei
NaCl 2.5-3fold increase in activity at 0.1 M Trypanosoma brucei
Urea stimulates 1.5fold at 0.75-1.5 M Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
methotrexate
-
Trypanosoma brucei
pyrimethamine
-
Trypanosoma brucei
trimethoprim
-
Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
7,8-dihydrofolate recombinant bifunctional enzyme Trypanosoma brucei
0.0009
-
NADPH recombinant bifunctional enzyme Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
trimethoprim-resistant strain
-
Trypanosoma brucei
-
bifuntional enzyme with dihydrofolate reductase and thymidylate synthase activity
-

Purification (Commentary)

Purification (Comment) Organism
recombinant bifunctional protein from Escherichia coli Trypanosoma brucei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12
-
recombinant bifunctional enzyme Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydrofolate + NADPH
-
Trypanosoma brucei 5,6,7,8-tetrahydrofolate + NADP+
-
?

Synonyms

Synonyms Comment Organism
DHFR-TS
-
Trypanosoma brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5
-
7,8-dihydrofolate recombinant bifunctional enzyme Trypanosoma brucei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 8 broad maximum, recombinant bifunctional enzyme Trypanosoma brucei

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Trypanosoma brucei

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information overview Trypanosoma brucei
0.00000023
-
methotrexate recombinant bifunctional enzyme Trypanosoma brucei
0.00000028
-
pyrimethamine recombinant bifunctional enzyme Trypanosoma brucei