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Literature summary for 1.5.1.3 extracted from

  • Rao, K.N.
    Purification and characterization of dihydrofolate reductase from Lactobacillus leichmannii (2000), Indian J. Biochem. Biophys., 37, 121-129.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
CaCl2
-
Lactobacillus leichmannii
KBr
-
Lactobacillus leichmannii
KCl 0.1 M Lactobacillus leichmannii
KI
-
Lactobacillus leichmannii
MgCl2
-
Lactobacillus leichmannii
Urea activates at 2 M about 1.5fold Lactobacillus leichmannii

Inhibitors

Inhibitors Comment Organism Structure
deoxytubulosine
-
Lactobacillus leichmannii
methotrexate competitive Lactobacillus leichmannii
p-chloromercuribenzoate
-
Lactobacillus leichmannii
pegularinine plant alkaloid Lactobacillus leichmannii
tylophorinidine plant alkaloid Lactobacillus leichmannii
Urea at concentration 3-4 M complete inactivation Lactobacillus leichmannii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0035
-
NADPH
-
Lactobacillus leichmannii
0.0062
-
7,8-dihydrofolate
-
Lactobacillus leichmannii

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2
-
Lactobacillus leichmannii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
gel filtration Lactobacillus leichmannii

Organism

Organism UniProt Comment Textmining
Lactobacillus leichmannii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lactobacillus leichmannii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
200
-
purified enzyme Lactobacillus leichmannii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydrofolate + NADPH
-
Lactobacillus leichmannii 5,6,7,8-tetrahydrofolate + NADP+
-
ir

Subunits

Subunits Comment Organism
monomer 1 * 20000, SDS-PAGE Lactobacillus leichmannii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Lactobacillus leichmannii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
30% loss of activity after 60 min Lactobacillus leichmannii
45
-
90% loss of activity after 30 min Lactobacillus leichmannii
50
-
90% loss of activity after 5 min Lactobacillus leichmannii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Lactobacillus leichmannii

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Lactobacillus leichmannii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000000007
-
methotrexate
-
Lactobacillus leichmannii
0.005
-
deoxytubulosine
-
Lactobacillus leichmannii
0.007
-
tylophorinidine
-
Lactobacillus leichmannii
0.009
-
pegularinine
-
Lactobacillus leichmannii