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Literature summary for 1.5.1.3 extracted from

  • Delcamp, T.J.; Susten, S.S.; Blankenship, D.T.; Freisheim, J.H.
    Purification and characterization of dihydrofolate reductase from methotrexate-resistant human lymphoblastoid cells (1983), Biochemistry, 22, 633-639.
    View publication on PubMed
Search for more literature for 1.5.1.3

Activating Compound

Activating Compound Comment Organism Structure
KCl
-
 Homo sapiens
p-chloromercuribenzoate activates in presence of NADPH Homo sapiens
p-hydroxymercuribenzoate
-
 Homo sapiens
Thiourea
-
 Homo sapiens
Urea
-
 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
methylmercuric hydroxide
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information overview Homo sapiens
0.036
-
 7,8-dihydrofolate
-
 Homo sapiens
0.25
-
 NADPH
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
K+ stimulation Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 methotrexate-resistant WIL-2 lymphoblastoid cells
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-
WIL-2 cell WIL2 lymphoblastoid cells Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.1
-
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 3 optima: pH 4.0, pH 7.3-7.5 and pH 8.3-8.5 Homo sapiens
7.3 7.5 3 optima: pH 4.0, pH 7.3-7.5 and pH 8.3-8.5 Homo sapiens
8.3 8.5 3 optima: pH 4.0, pH 7.3-7.5 and pH 8.3-8.5 Homo sapiens