Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methotrexate | cell line HeLa BU-25 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | 0.007 | folate | - |
Homo sapiens | |
0.165 | - |
NADPH | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
methotrexate-sensitive cell line HeLa BU-25, methotrexate-insensitive cell line VA2-B | - |
Homo sapiens | - |
methotrexate-resistant WIL-2 lymphoblastoid cells | - |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | HeLa cells/BU-25, normal and methotrexate-resistant variant, methotrexate variant of VA2-B cell line | Homo sapiens | - |
WIL-2 cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | enzyme content of methotrexate-insensitive cell line VA2-B is 200fold higher than that of sensitive cell line HeLa BU-25 due to production of alternate enzyme type | Homo sapiens | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
folate + NADPH | - |
Homo sapiens | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.08 | - |
folate | - |
Homo sapiens | |
8.33 | - |
7,8-dihydrofolate | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.2 | 5.6 | half-maximal activities at pH 4.2 and pH 5.6 | Homo sapiens |