Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.1.3 extracted from

  • Smith, S.L.; Patrick, P.; Stone, D.; Phillips, A.W.; Burchall, J.J.
    Porcine liver dihydrofolate reductase. Purification, properties, and amino acid sequence (1979), J. Biol. Chem., 254, 11475-11484.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
KCl 3.5fold activiation at 0.5 M Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
methotrexate
-
Sus scrofa
NADP+
-
Sus scrofa
tetrahydrofolate product inhibition Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00074
-
7,8-dihydrofolate
-
Sus scrofa
0.00322
-
NADPH
-
Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22500
-
gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
35.8
-
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydrofolate + NADPH
-
Sus scrofa 5,6,7,8-tetrahydrofolate + NADP+
-
?
folate + NADPH slow rate Sus scrofa 5,6,7,8-tetrahydrofolate + NADP+
-
?
folate + NADPH activity only at acidic pH around 4.5 Sus scrofa 5,6,7,8-tetrahydrofolate + NADP+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 22500, SDS-PAGE Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.5
-
7,8-dihydrofolate
-
Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5.5 2 optima: pH 4.5-5.5 and pH 7.6 Sus scrofa
7.6
-
2 optima: pH 4.5-5.5 and pH 7.6 Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0023
-
NADP+ with 7,8-dihydrofolate Sus scrofa