Activating Compound | Comment | Organism | Structure |
---|---|---|---|
KCl | 3.5fold activiation at 0.5 M | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methotrexate | - |
Sus scrofa | |
NADP+ | - |
Sus scrofa | |
tetrahydrofolate | product inhibition | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00074 | - |
7,8-dihydrofolate | - |
Sus scrofa | |
0.00322 | - |
NADPH | - |
Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22500 | - |
gel filtration | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
35.8 | - |
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | - |
Sus scrofa | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
folate + NADPH | slow rate | Sus scrofa | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
folate + NADPH | activity only at acidic pH around 4.5 | Sus scrofa | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 22500, SDS-PAGE | Sus scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.5 | - |
7,8-dihydrofolate | - |
Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5.5 | 2 optima: pH 4.5-5.5 and pH 7.6 | Sus scrofa |
7.6 | - |
2 optima: pH 4.5-5.5 and pH 7.6 | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
NADP+ | with 7,8-dihydrofolate | Sus scrofa |