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Literature summary for 1.5.1.3 extracted from
- Purification, properties, and synthesis of dihydrofolate reductase from wild type and methotrexate-resistant hamster cells (1972), J. Biol. Chem., 247, 179-187.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| methotrexate | - |
Cricetulus sp. |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Cricetulus sp. |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
sucrose density gradient centrifugation | Cricetulus sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cricetulus sp. | - |
wild type and methotrexate-resistant | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| wild type and methotrexate-resistant cells | Cricetulus sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Cricetulus sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Cricetulus sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Cricetulus sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | NADPH 16fold more active than NADH | Cricetulus sp. | |
| NADPH | NADPH 16fold more active than NADH | Cricetulus sp. | |
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