Any feedback?
Literature summary for 1.5.1.3 extracted from
- A soluble dihydrofolate reductase from human placenta: purification and properties (1971), Arch. Biochem. Biophys., 142, 417-425.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Cl- | - |
Homo sapiens |  |
| Urea | - |
Homo sapiens | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-(N-[(2,4-Diamino-6-pteridyl)methyl]-N-methylamino)benzoate | - |
Homo sapiens | |
| aminopterin | - |
Homo sapiens | |
| Dichloromethotrexate | - |
Homo sapiens | |
| folate | - |
Homo sapiens | |
| methotrexate | dihydrofolate antagonist drug | Homo sapiens | |
| p-hydroxymercuribenzoate | - |
Homo sapiens | |
| Pteroate | - |
Homo sapiens | |
| pyrimethamine | - |
Homo sapiens | |
| Triampterene | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | stimulation | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | 22000 | human placenta, gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH | Homo sapiens | key role in thymidylate biosynthesis | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
methotrexate-resistant WIL-2 lymphoblastoid cells | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| placenta | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
| 17.5 | - |
purified enzyme | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% glycerol, 0.1 M potassium phosphate, pH 7.0, less than 10% loss of activity, 6 months | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH | - |
Homo sapiens | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| 7,8-dihydrofolate + NADPH | key role in thymidylate biosynthesis | Homo sapiens | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| folate + NADPH | - |
Homo sapiens | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.63 | - |
7,8-dihydrofolate | pH 6.0 | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | - |
2 optima: pH 4.2 and pH 7.5 | Homo sapiens |
| 7.5 | - |
2 optima: pH 4.2 and pH 7.5 | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | low activity | Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | overview | Homo sapiens | |
| 0.0000000061 | - |
methotrexate | - |
Homo sapiens | |
| 0.0000000078 | - |
aminopterin | - |
Homo sapiens | |
| 0.00000078 | - |
4-(N-[(2,4-Diamino-6-pteridyl)methyl]-N-methylamino)benzoate | - |
Homo sapiens | |
| 0.0000022 | - |
pyrimethamine | - |
Homo sapiens | |
| 0.00002 | - |
Triampterene | - |
Homo sapiens | |
| 0.000026 | - |
folate | - |
Homo sapiens | |
| 0.0018 | - |
Pteroate | - |
Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
